Bacterial Growth

Question 1

Through what method do bacteria grow?

Answer 1

Binary Fusion

Question 2

Generation time

Answer 2

time required for bacteria to give rise to 2 daughter cells under optimum conditions

Question 3

When graphing large magnitude of bacteria populations what method is best to use and why?

Answer 3

Best to use logarithmic logging

Question 4

List the Phases of Growth for bacteria

Answer 4

1. Lag Phase
2. Log or Exponential Phase
3. Stationary Phase
4. Phase of Decline

Question 5

Lag Phase

Answer 5

Bacteria are metabolically active but not yet replicating. their is increase in cell size

Question 6

Log Phase or Exponential Phase

Answer 6

s increase rapidly

exponential growth. cells divide rapidly

Question 7

Stationary Phase

Answer 7

• Cell division stops due to depletion of nutrients and toxic product build up
• equilibrium between dying cells and newly formed cells
• growths reach plateau

Question 8

Phase Decline Phase

Answer 8

Decrease in # of cells due to decrease in dying cells

autolytic enzymes

Question 9

What is Batch Culture?

Answer 9

a closed system where cells are grown in specific environmental conditions, up to certain density in a tank or airlift fermentor

Question 10

What is Continuous Culture?

Answer 10

A Chemostate is used. it functions by having a continuous flow of fresh medium while having old medium, which includes microbes, removed

• it is an open system
• keeps cells in specific phase