Deck 13 Flashcards
1
Q
Fujiwar’s reaction is used in the determination of
A
chlorinated aliphatic hydrocarbons (creates pink or red ring)
2
Q
What kind of compounds do we use from steam distillation?
A
- aldehydes (formaldehyde)
- cyanides
- hydrogen phosphide
- chlorinated aliphatic hydrocarbons
- phenols
- cresols
3
Q
Division of glycosides
A
cyanide and other N-organic compounds
4
Q
Benzidine test is used
A
in qualitative determination of cyanide (blue colour)
5
Q
Explain the TLC method
A
- TLC is performed on a sheet of glass, plastic or aluminium foil coated w/absorbent material. Known as the stationary phase. Absorbent material can be silica gel, aluminium oxide or cellulose
- Small spot of solvent containing the sample is added to the plate, 1 - 1.5cm from bottom edge
- An appropriate solvent is poured into the glass to a depth less than 1cm. The plate with sample is put into the chamber, so bottom touches the solvent. Chamber is closed with a lid & left for a few minutes, so the solvent can be drawn up the plate by capillary action (mobile phase)
- When the solvent mixture has been drawn up closely to the top, it is taken out from the chamber
- Sample plate is dried off and observed in visible or UV light
- Visible spots are measured & compared with the other spots. The different analytes will move along the TLC plate at different rates
6
Q
Supermetrin is determined by
A
GC, extracted in organic solvent
7
Q
What do we use as treatment in organophosphate intoxication?
A
atropine
8
Q
Which methods we use for determination of organophosphate in biological material?
A
TLC & GC –> Bromthymol blue
9
Q
What kind of chromatographic methods do you know?
A
- TLC (thin layer chromatography
- HPLC (high pressure chromatography)
- GC (gas chromatography)
10
Q
Treatment of paracetamol poisoning
A
ascorbic acid, gastric lavage, charcoal
- Antidote : N-acetylcysteine
