CSF: Intro to the cell Flashcards
cell theory (3pts)
- all living organisms are composed of one or more cells
- the living cell is the basic unit of structure and organisation
- all cells arrise from preexisting cells
The central Dogma equation
DNA -> RNA -> PROTEIN
what are 6 things prokaryote AND eukaryote cells have
Plasma membrane, cystol, DNA, RNA, protein and ribosomes
Prokaryote nucleases are not…
membrane bound
what are two things that make eukaryote different than pro?
- organelles are membrane-bound
2. they are larger than prokaryote
what is the cytoplasm/what does it contain
everything inside plasma membrane including organelles, not the nucleus though
what is the fluid portion of the cytoplasm called and made up of
the cystol
water + dissolved and suspended substances - ions, ATP, proteins, lipids etc
what is the function of the plasma membrane
its a physical barrier seperating the inside/outside of the cell
whats the general function of the plasma membrane
double layer of phospholipids with embedded proteins
what does hydrophilic mean
loves water
what does hydrophobic mean
hates water aka fats therefore, lipids in membrane provide a barrier to water
what makes up the head of a phospholipid
a hydrophilic phosphate head (polar)
what makes up the tail of a phospholipid
a hydrophobic lipid tail
what word describes hydrophilic and hydrophobic sections in plasma membrane proteins
amphipathic
describe intergral proteins
imbedded - partially or fully - into the membrane
what are transmembrane proteins
- fulls span the entire membrane
- contact extracellular and cytoplasmic areas
What are peripheral membrane proteins
- associated with the membrane but not actually embedded in it
what do TRANSPORT plasma membrane proteins do?
channels, transporters may be general or selective, gated or not
what do ENZYMES plasma membrane proteins do?
- carry out chemical reaction, possibly part of enzyme team
what do SIGNAL TRANSDUCTION plasma membrane proteins do?
- external signalling molecule
- transduction of info to cell inside
what do CELL-CELL RECOGNITION plasma membrane proteins do?
- glycoproteins as molecular signatures on the extracellular side of cell
what do INTERCELLULAR plasma membrane proteins do?
e.g gap junctions or tight junctions
what do Attachment to the cytoskeleton and ECM plasma membrane proteins do?
- fibroconectin helps contact between cell surface integrins and ECM (e.g. collagen)
- can facilitate movement
whats the best model to describe plasma membrane
fluid mosaic model
Whats the nucleus enclosed in
double lipid layer nuclear envelope, continuous with rough ER
how does shit get in and out of the nucleus
through nuclear pores
what happens in the nucleolus
- rRNA production
- assembly of small and large subunits of ribosomes
Three functions of DNA and its pores
- house/protect DNA
- make RNA & assemble ribosomes
- pores regulate substance movement in and out
whats so great about molecule segregation?
- molecule segregation to allow temporal and spatial control of cell function
what does DNA code for?
our phenotype
What proteins is DNA wrapped around to pack is densely
it wraps twice around a group of 8 histones
when DNA wraps twice around a group of 8 histones what is this called
it forms nucleosomes or chromatin
what does chromatin condense down to to become a chromosome?
Chromatin fiber condenses further into loops to become a chromosome.
Chomosome idea
comprised of many genes
gene idea
a DNA segment that contributes to phenotype/function
Humans are diploid, what does this mean?
2N = 46
- 23 pairs of choromosomes, (1 from each parent
- 22 autosomes
- 2 sex chromosomes
how far does DNA stretch out to
2 meters
accessibility is determined by what
extent of coiling
ribosome structure
2 subunits, burger - top big bottom small, made of ribosomal RNA (rRNA) in protein complex
where is Rrna made
nucleolus