CSF: Intro to the cell Flashcards

1
Q

cell theory (3pts)

A
  1. all living organisms are composed of one or more cells
  2. the living cell is the basic unit of structure and organisation
  3. all cells arrise from preexisting cells
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2
Q

The central Dogma equation

A

DNA -> RNA -> PROTEIN

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3
Q

what are 6 things prokaryote AND eukaryote cells have

A

Plasma membrane, cystol, DNA, RNA, protein and ribosomes

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4
Q

Prokaryote nucleases are not…

A

membrane bound

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5
Q

what are two things that make eukaryote different than pro?

A
  1. organelles are membrane-bound

2. they are larger than prokaryote

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6
Q

what is the cytoplasm/what does it contain

A

everything inside plasma membrane including organelles, not the nucleus though

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7
Q

what is the fluid portion of the cytoplasm called and made up of

A

the cystol

water + dissolved and suspended substances - ions, ATP, proteins, lipids etc

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8
Q

what is the function of the plasma membrane

A

its a physical barrier seperating the inside/outside of the cell

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9
Q

whats the general function of the plasma membrane

A

double layer of phospholipids with embedded proteins

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10
Q

what does hydrophilic mean

A

loves water

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11
Q

what does hydrophobic mean

A

hates water aka fats therefore, lipids in membrane provide a barrier to water

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12
Q

what makes up the head of a phospholipid

A

a hydrophilic phosphate head (polar)

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13
Q

what makes up the tail of a phospholipid

A

a hydrophobic lipid tail

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14
Q

what word describes hydrophilic and hydrophobic sections in plasma membrane proteins

A

amphipathic

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15
Q

describe intergral proteins

A

imbedded - partially or fully - into the membrane

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16
Q

what are transmembrane proteins

A
  • fulls span the entire membrane

- contact extracellular and cytoplasmic areas

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17
Q

What are peripheral membrane proteins

A
  • associated with the membrane but not actually embedded in it
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18
Q

what do TRANSPORT plasma membrane proteins do?

A

channels, transporters may be general or selective, gated or not

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19
Q

what do ENZYMES plasma membrane proteins do?

A
  • carry out chemical reaction, possibly part of enzyme team
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20
Q

what do SIGNAL TRANSDUCTION plasma membrane proteins do?

A
  • external signalling molecule

- transduction of info to cell inside

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21
Q

what do CELL-CELL RECOGNITION plasma membrane proteins do?

A
  • glycoproteins as molecular signatures on the extracellular side of cell
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22
Q

what do INTERCELLULAR plasma membrane proteins do?

A

e.g gap junctions or tight junctions

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23
Q

what do Attachment to the cytoskeleton and ECM plasma membrane proteins do?

A
  • fibroconectin helps contact between cell surface integrins and ECM (e.g. collagen)
  • can facilitate movement
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24
Q

whats the best model to describe plasma membrane

A

fluid mosaic model

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25
Q

Whats the nucleus enclosed in

A

double lipid layer nuclear envelope, continuous with rough ER

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26
Q

how does shit get in and out of the nucleus

A

through nuclear pores

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27
Q

what happens in the nucleolus

A
  • rRNA production

- assembly of small and large subunits of ribosomes

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28
Q

Three functions of DNA and its pores

A
  • house/protect DNA
  • make RNA & assemble ribosomes
  • pores regulate substance movement in and out
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29
Q

whats so great about molecule segregation?

A
  • molecule segregation to allow temporal and spatial control of cell function
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30
Q

what does DNA code for?

A

our phenotype

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31
Q

What proteins is DNA wrapped around to pack is densely

A

it wraps twice around a group of 8 histones

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32
Q

when DNA wraps twice around a group of 8 histones what is this called

A

it forms nucleosomes or chromatin

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33
Q

what does chromatin condense down to to become a chromosome?

A

Chromatin fiber condenses further into loops to become a chromosome.

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34
Q

Chomosome idea

A

comprised of many genes

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35
Q

gene idea

A

a DNA segment that contributes to phenotype/function

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36
Q

Humans are diploid, what does this mean?

A

2N = 46

  • 23 pairs of choromosomes, (1 from each parent
  • 22 autosomes
  • 2 sex chromosomes
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37
Q

how far does DNA stretch out to

A

2 meters

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38
Q

accessibility is determined by what

A

extent of coiling

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39
Q

ribosome structure

A

2 subunits, burger - top big bottom small, made of ribosomal RNA (rRNA) in protein complex

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40
Q

where is Rrna made

A

nucleolus

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41
Q

how does rRNA get made/leave nucleus

A

subunits assemble in nucleolus, leave through nuclear pores

42
Q

function of ribosomes (protein)

A
  • protein production (translation)
43
Q

function of ribosomes (free))

A

making proteins to be used in cystol (non-endomembrane destinations)

44
Q

function of ribosomes (RER)

A

making non-cytosolic proteins/endomembrane

45
Q

what is the ER

A

extensive network of tubes and tubules - stretches out from nuclear membrane

46
Q

two types of ER

A

smooth ER and rough ER

47
Q

RER description

A

continuous with nuclear envelope dotted with attached ribosomes

48
Q

how do proteins and ER get folded?

A

proteins enter lumen within ER for folding

49
Q

3 major functions of RER

A

production of proteins:

  • secreted
  • membrane
  • organelle
50
Q

how does RER send things to golgi

A

RER membrane surrounds protein to form transport vesicles destined for golgi

51
Q

smooth ER location

A

extends from rough ER

52
Q

does the smooth ER have ribosomes?

A

no, it doesnt make proteins

53
Q

three major function of smooth ER

A
  • housing unit for proteins and enzymes
  • synthesizes lipids - steroids and phospholipids
  • storage of cell-specific proteins, not all cells make all proteins
54
Q

are the smooth ER functions the same for every cell?

A

no, very cell/tissue specific

55
Q

what does the smooth ER do in the liver?

A

houses enzymes for detoxification and for glucous release

56
Q

what does the smooth ER do in the muscles?

A

calcium ions

57
Q

what is a colloquial term for the golgi apparatus

A

the ‘warehouse’

58
Q

how many sacs is the golgi made of and what are they called? (golgi description)

A

3-20 flattened membranous sacs called cisternae stacked on top of one another

59
Q

functions of golgi

A
  • modify, sort, package and transport proteins from RER vis enzymes in cisternae
60
Q

golgi assists in the formation of

A
  • secretory vesicles
  • membrane vesicles
  • transport vesicles
61
Q

secretory vesicles golgi used for

A

proteins for exocytosis

62
Q

membrane vesicles golgi used for

A

PM molecules

63
Q

transport vesicles golgi used for

A

molecules to lysosome

64
Q

two golgi faces and uses of those faces

A

cis face - receiving side closer to ER

trans face - shipping side further from ER

65
Q

each sac or cisternae contains what?

A

enzymes of different functions

66
Q

protein movement in golgi 1

A

-proteins move from cis to trans (sac to sac)

67
Q

protein movement in golgi 2

A
  • mature at exit cisternae
68
Q

protein movement in golgi 3

A
  • travel to destination
69
Q

what occurs in each golgi sac?

A

modifications - formation of glycoproteins, glycolipids and lipoproteins

70
Q

lysosome contain

A

powerful digestive enzymes

71
Q

are lysosomes acidic or basic?

A

highly acidic, pump H+ to maintain acidic pH

72
Q

main function of lysosomes is digestion of:

A
  • substances that enter a cell
  • cell components e.g. organelles - autography
  • entire cells - autolysis
73
Q

cell components e.g. organelles name

A

autography

74
Q

entire cells name

A

autolysis

75
Q

mitochondria main function:

A

generation of ATP through cellular respiration

76
Q

three things mitochondria are made up of

A
  • outer mitochondrial membrane
  • inner mitochondrial membrane
  • fluid filled interior cavity the mitochondrial matrix
77
Q

why do cells have more ATP

A

when a cell requires more energy itll have more ATP

78
Q

what special thing does mitochondria carry

A

separate small (37 gene) genome encoding mitochondria specific products

79
Q

what is ATP

A

adenosine triphosphate - our energy currency

80
Q

how do we get energy in terms of product transfer

A

one phosphate transfers to another molecule and provides energy

81
Q

Cytoskeleton idea

A

structural support system of the cell

82
Q

three fibres in cytoskeleton

A
  • microfilmanets
  • intermediate filaments
  • microtubules
83
Q

smallest cytoskeleton fiber

A

microfibers

84
Q

medium cytoskeleton fibers

A

intermediate filaments

85
Q

largest cytoskeleton fibres

A

microtubules

86
Q

cytoskeleton fibres and filamentss help maintain what of the cell

A

size, shape and integrity of cell

87
Q

two main cytoskeleton jobs

A
  • act as scaffolding across the cell

- involved in intracellular transportation and cell movement

88
Q

microfilament diameter

A

7nm

89
Q

what are microfilaments comprised of

A

actin molecules - 2 long chains twisted

90
Q

where are microfilaments found

A

periphery of interior of cell

91
Q

function of microfilaments

A
  • anchor cytoskeleton to plasma membrane proteins

- promote amoeboid mobility

92
Q

Intermediate filaments diameter

A

8-12nm

93
Q

intermediate filaments composition

A

keratin

94
Q

intermediate filament location

A

cytoplasm

95
Q

which is the most permanent cytoskeleton

A

intermediate

96
Q

function of intermediate filmants

A
  • scaffold for cellular organs i.e. nucleus

- bear tension and weight

97
Q

microtubules diameter

A

25nm

98
Q

microtubules composition

A

tubulin dimers (alpha and beta) coiled to form a tube

99
Q

4 functions of microtubules

A
  • support cell type
  • guide for organelle movement
  • chromosome organisation - cell division
  • support and movement of cillia/flagella
100
Q

are microfilaments dynamic?

A

yes they are dynamic - assemble and dissemble as required

101
Q

are microtubules dynamic?

A

yes they are dynamic - assemble and dissemble as required