Coronaviridae Flashcards
Choose the INCORRECT association for Coronaviridae:
A. Enveloped virions with club-shaped spike proteins
B. Nucleocapsid structure is EITHER a helical shape or tightly coiled tubular one bent into doughnut shape
C. Icosahedral internal core structure
D. Virions are produced via budding through the plasma membrane
D. is not correct- after replication, the virus buds from the endoplasmic reticulum (ER) and released from the host cell via EXOCYTOSIS.
So, Coronaviridae is enveloped bc when you drink a Corona you want to cover it with a coozie. It is positive-sense because Corona is GOOD (beer is good). Club-shaped spikes= you drink Corona in the club.
Budding thru the ER, bc if you and your buddies drink too much Corona, you’ll wind up in the ER!
T/F:
Genus Torovirus possesses the helical nucleocapsid, whereas the genus Coronavirus possesses the tightly coiled tubular nucleocapsid that is bent into the shape of a doughnut.
F.
Torovirus=tubular doughnut nc
Coronavirus= helical
I think of it as drinking Corona from an ice luge, you are going to have a lot of helical turns; if you eat too many doughnuts, someone is going to hold up a red cloth and yell “Toro” at you lol
T/F:
Porcine Epidemic Diarrhea is a deletion mutant of Transmissable Gastroenteritis in pigs.
False!!!!
Porcine Respiratory Corona Virus is a deletion mutant of Transmissible Gastroenteritis of Pigs
Which of the following is TRUE of Transmissable Gastroenteritis Virus (TGEV) in pigs?
A. It is an OIE List A disease
B. It is in genus Coronavirus, Group 1a
C. Causes severe GI signs in all age ranges of pigs
D. The Epidemic form is associated with partially immune herds or with co-infection with Bovine Respiratory Coronavirus.
E. The Endemic form is associated with first introduction of virus into a susceptible herd, usually in winter.
B. Genus Coronavirus 1a
OIE list B
Severe disease in PIGLETS
Endemic form= less severe signs, partially susceptible herds co-infected with BRCV
Epidemic= Winter, first introduction
T/F:
Although Porcine Epidemic Diarrhea causes similar clinical signs as Transmissible Gastroenteritis in Pigs, the diseases are unrelated, with the former in the genus Coronavirus group 1a and the latter in group 1b
False
TGEV=1a
PEDV=1b
T/F:
Porcine Hemagglutinating Encephalomyelitis (Vomiting and Wasting Disease in pigs) has two forms: acute encephalomyelitic (seen in pigs
True
Which of the following is NOT part of the pathogenesis of the WASTING form of Hemagglutinating Encephalomyelitis in pigs?
A. Replication of virus in ganglion distale vagi, causing vomiting
B. Disturbance of gastric emptying due to replication in vagal ganglion and intramural plexi of stomach
C. Replication in cerebellum, brainstem and cerebrum
D. All of the above are correct
D.
All of the following are characteristics of Bovine Coronavirus infection except:
A. Infects calves under 3 months, usually 1-2 weeks old.
B. More common to see in winter months
C. Virus replicates in GI and respiratory tracts
D. Especially watery diarrhea, leading to severe dehydration
E. Outbreaks usually affect relatively few animals in a herd.
E.
EXPLOSIVE outbreaks! FAST ONSET!!!
EXPLOSIVE DIARRHEA!
Vaccinations for Bovine Coronavirus will often be in combination with the following diseases:
A. Foot-and-mouth B. Bluetongue C. E. coli D. Rotavirus E. C and D only
E.
Rotavirus, E. coli and Corona virus are important enteric pathogens for cattle, and they combine them into a vaccine. Plus he LOVES rotavirus so he could definitely ask this.
T/F:
The main difference between Bovine Coronavirus and Winter Dysentery in Cattle is that BCV is a severe disease of young calves, whereas WD tends to affect mature cattle more severely (particularly post-partum lactating cows).
True
Which of the following is NOT true of Feline Infectious Peritonitis?
A. It is caused by feline Coronavirus
B. Its main mode of transmission is via fecal-oral, but can also be inhaled and can cross the placenta
C. Only about 1-3% of cats that become infected will develop serious disease.
D. Most cats that become infected will become chronic carriers
D.
Most cats (70%) will develop a mild enteric disease that is transient, but they are not known to remain as carriers
5-10% of cats will become chronic carriers
5-10% of cats will be completely resistant to the virus
Which of the following is the MISMATCHED pair for FIP?
A.Strong cell-mediated response (CMI) only: clear disease
B. Strong CMI and strong Humoral responses: Effusive/wet FIP
C. Intermediate response: Dry FIP
D. Strong humoral but weak CMI: wet/effusive FIP
B. Strong CMI and humoral- he does not say what would happen
Strong HUMORAL but weak CMI responses result in wet FIP. Antigen-Antibody complexes deposit into vessel walls–> complement activation—>vasculitis—> leaky vessels—> EDEMA!!
T/F:
Antibodies to FIPV spike protein enhance virus uptake into macrophage and subsequent replication inside the macrophage
True!
The antibodies are NOT effective at neutralizing the virus…in fact, they help the VIRUS!
T/F:
Macrophages have a receptor for FIPV, called CD-13, which binds to the virus spike protein.
True!
Which of the following are characteristics of the DRY form of FIP:
A. Granumomatous foci in CNS or in other places
B. Pyogranulomatous foci in abdominal viscera
C. Granulomatous uveitis
D. Polyserositis
E. A and C only
E
Dry= mostly eyes and CNS, but granulomatous lesions can be elsewhere like kidney and LNs
Wet= serofibrinous polyserositis, pyogranulomatous foci in viscera, edema
