Citric Acid Cycle Flashcards

1
Q

Where does the citric acid cycle occur?

A

Matrix of the mitochondria.

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2
Q

Why is the citric acid cycle very efficient?

A

Does not need many intermediate compounds.

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3
Q

What does the citric acid cycle act as a gateway for?

A

Any compound that can be converted into acetyl coenzyme A or a component of the cycle to enter aerobic respiration.

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4
Q

How does the citric acid cycle produce ATP?

A

Not directly, but through the use of electron carriers that then go to the electron transport chain and produce ATP.

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5
Q

What does the citric acid cycle do?

A

Removes electrons from intermediates and passes them onto NADH and FADH.

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6
Q

Is O2 used in the citric acid cycle?

A

NO

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7
Q

What does the use of O2allow for glucose?

A

It to be completely broken down into CO2and all of its energy to be stored.

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8
Q

What substances can be broken down into acetyl coenzyme A and enter the citric acid cycle?

A

*Glucose
*Fatty acids
*Amino acids

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9
Q

What does the overall metabolism process look like (glycolysis, citric acid cycle and the electron transport chain)?

A

And electron transport chain gives 30-32 atp*

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10
Q

Why are substances like glucose broken down in lots of little steps and not all at once?

A

It allows packets of energy to be stored, breaking down the compound at once would release to much enery to be capture so most of it would be lost as heat.

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11
Q

What does acetyl coenzyme A allow?

A

Many intermediates to enter the citric acid cycle.

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12
Q

What enzyme coverts pyruvate into acetly coenzyme A?

A

Pyruvate dehydrogenase complex.

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13
Q

How many sub units is pyruvate dehydrogenase complex composed of?

A

TEN copies of each sub unit(E1+ E2+ E3)

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14
Q

What does each subunit of pyruvate dehdrogenase complex do?

A

Catalyses a different part of the reaction.

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15
Q

What happens to the concentration of the intermediates within the citric acid cycle?

A

They remain constant as each turn 2 carbons enter (acetyl coenzyme A) and 2 carbons leave (as 2 CO2).

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16
Q

What does the citric acid cycle look like in terms of the amount of carbon each complex has and the use of electron carriers?

A
17
Q

What are the intermediates of the citric acid cycle?

A
  1. citrate
  2. iso-citrate
  3. alpha-ketoglutarate
  4. succinate
  5. fumarate
  6. malate
  7. oxaloacetate
18
Q

What do intermediate products of the citric acid cycle and ATP do to pyruvate dehydrogenase complex?

A

Regulate it.

19
Q

What substance inhibit (-ly regulate) pyruvate dehydrogenase complex?

A

Acetyl coenzyme
NADH
ATP

20
Q

What substances activate (+ly regulate) pyruvate dehydrogenase complex?

A

Pyruvate.
ADP.

21
Q

What does the regulation of pyruvate dehydrogenase complex mean?

A

Energy is not generated when it is not needed.

22
Q

What are the points of control in the citric acid cycle other than the inhibition of pyruvate dehdrogenase complex?

A

Regulating the irreversible steps.

23
Q

What enzymes control the irreversible steps of the citric acid cycle that can be regulated?

A

Isocitrate dehydrogenase and a-ketoglutamate dehydrogenase.

24
Q

What does ATP and NADH do to isocitrate dehyrogenase?

A

(Negatively regulate it) Inhibit it.

25
Q

What does ADP do to isocitrate dehydrogenase?

A

(Positively regulate it) Activate it.

26
Q

What does ATP, NADH and succinyl CoA do to a-ketoglutamate dehydrogenase?

A

(Negatively regulate it) Inhibit it.

27
Q

What does controlling the irreversible steps of the citric acid cycle allow for?

A

The build up of intermediates (citrate and a-ketoglutamate) which can be used elsewhere.

28
Q

What kind of pathway is the citric acid cycle?

A

Amphibolic as the intermediates can be used in other anabolic pathways.

29
Q

What does amphilbolic mean?

A

Serves both catabolic and anabolic processes.

30
Q

What can the citric acid cycle make building blocks for when energy levels are okay?

A

Nucleotide bases.
Heme groups.
Proteins.

31
Q

What is a problem with intermediates of the citric acid cycle being used elsewhere?

A

Can lead to a deficiency of the intermediates, stopping the citric acid cycle and the generation of energy.