Chapter 5: Histology Flashcards
Four categories of tissues
Epithelial
Connective
Nervous
Muscular
Organ
Structure with discrete boundaries composed of two or more tissue types
Tissue
Group of similar cells and cell products working together to perform a specific role in an organ
The matrix
Extracellular material
Composed fibrous proteins, clear gel called ground substance
Aka tissue fluid, ECF, interstitial fluid or tissue gel
Three germ layers
Ectoderm - outer layer
Mesoderm - middle layer
Endoderm - inner layer
Ectoderm
Outer
Gives rise to epidermis and nervous system
Mesoderm
Middle
Wispy collagen fibers and fibroblasts in gel matrix
Gives rise to cartilage, bone, blood
Endoderm
Gives rise to mucous membrane lining digestive and respiratory tracts
Formalin
Fixative prevents decay of tissue for slides
Histological sections
Tissue is sliced into thin sections one or two cells thick
Stains
Tissue is mounted on slides and artificially colored with histological stain
Longitudinal section cut
Tissue cut on its long axis
Cross section
Tissue cut perpendicular to long axis of organ
Oblique section
Tissue cut at angle between cross and longitudinal sections
Smear
Tissue rubbed across a slide
Spread
Some membranes and cobwebby tissues are laid out on a slide
Epithelial tissue
> = 1 cell thick
Covers body surface and lines body cavity
Upper surface exposed to the environment or an internal space
Constitutes most glands
Avascular
Avascular
Does not have blood vessels
Functions of epithelial tissue
Protect
Secretes chemical
Excrete waste
Absorb chemicals
Filter substance
Sense stimuli
Simple epithelia
One layer
Named by shape of cells
All cells touch basement membrane
Stratified epithelia
Contain more than one layer
Apical cells
Some rest on top of other and do not touch basement membrane
Four types of simple epithelia
Simple squamous
Simple cuboidal
Simple columnar
Pseudostratified columnar
(See PowerPoint for photos and description of each)
Goblet cells
Mucus secreting cells in simple columnar and pseudostratified epithelia
(See PowerPoint for photos)
Four types of stratified epithelia
Three names for their shape of their apical surface cells
Stratified squamous
Stratified cuboidal
Stratified columnar (rare)
Fourth type: transitional epithelium
(See PowerPoint for photos and description of each)
Stratified squamous
Most widespread epithelium in the body
Deepest layers undergo continuous mitosis
Daughter cells push toward the surface and become flatter
Finally die and flake off - exfoliate/desquamation
Two kinds: keratinized and non keratinized (See PowerPoint for photo and description)
Connective tissue
Diverse abundant type of tissue in which cells occupy less space than the matrix
Most cells are not in direct contact with each other
Function: support, connect and protect organs
Highly variable vascularity
8 Functions of connective tissue
Connect organs - tendons and ligmanents
Support - bones and cartilage
Protect - cranium, ribs, sternum
Immune protection - WBC
Movement - bones provide lever system
Storage - fat, calcium, phosphorous
Heat production- metabolism of brown fat
Transport - blood
Four types of connective tissue
Fibrous ( loose and dense)
Adipose
Supportive (bone)
Fluid (blood)
(Check PowerPoint for dichotomous key of CT)
6 cells of fibrous connective tissue
Fibroblasts - produce fiber and ground substance
Macrophages- (antigens) get rid of foreign material
Leukocytes- WBC (neutrophils, lymphocytes)
Proteins
Mast cells- inhibit clotting, secrete histamine
Adipocytes- store triglycerides
3 fibers of fibrous CT
Collagenous
Reticular
Elastic
(PowerPoint for description of each)
Ground substance
- usually gelatinous
- made of glycosaminoglycans (GAGs), proteoglycans and adhesive glycoproteins
Types of fibrous connective tissue
Loose CT- mostly made up of ground substance (areolar and reticular)
Dense CT- mostly fibers (dense regular and dense irregular)
Adipose tissue
Adipocytes are the dominant cell type
Space between is occupied by areolar tissue, reticular tissue and blood capillaries
2 types of adipose tissue
White fat- found in adults, thermal insulation, cushions, body contours in females
Brown fat- found in fetuses, infants and children. Functions as heat generating tissue
Cartilage - 3 types, description and cells
Supportive CT = hyaline, elastic and fibrocartilage (See PPT for pictures and description)
Stiff connective tissue with flexible matrix, shapes ear, nose and larynx, avascular
Cells of cartilage- chondroblasts, chondrocytes
Bone/Osseous Tissue
Bone (osseous tissue) is calcified connective tissue
Two types of osseous tissue- spongy and compact (See PPT for photo and description)
Concentric lamallae
Onionlike layers around each canal of osseous tissue
Lacunae
Small cavity that contains an osteocyte
Osteon
Central canal and surrounding lamellae