Chapter 10: 10.6 Karyotyping

Question 1

Karyotype

Answer 1

Number, size and shape of chromosomes in a cell during metaphase

Question 2

What is a human karyotype comprised of?

Answer 2

• 22 pairs of autosomal chromosomes
• 1 sex chromosome pair

Question 3

Chromosome painting

Answer 3

A method used to idnetify and differentiate the chromosomes since many chromosomes have similar sizes

Question 4

FISH

Answer 4

Fluorescent in-situ hybridization
* A technique in which fluorescent probes that are specific for each chromosome are hybridized to the chromosomes so they can be identified

Question 5

What are some abnormalities that FISH can detect?

Answer 5

• Abnormal numbers of chromosomes (e.g. Down’s Syndrome)
• Deletions or duplications of chromosomal regions
• Determine how species are evolutionarily related