Chapter 1 - Safety in the Laboratory Flashcards
Potentially harmful microorganisms
Biological hazard
The chain of infection requires a continuous link between:
Source, mode transmission, susceptible host
6 components of the chain of infection
Infectious agent, reservoir, exit, means of transmission, entry portal, susceptible host
The best way to break the chain of infection
Handwashing
What is the primary method of infection transmission?
Hand contact
PPE stands for?
Personal protective equipment
When hands are visibly soiled, then…
Wash hands with soap and water
When hands are not visibly soiled then…
Apply alcohol based hand-rub (ex: sanitizer)
Handwashing procedure: wet hands with _____ water.
Warm
The most important part of handwashing.
Friction
Handwashing procedure: thoroughly clean between fingers, including thumbs, under fingernails and rings, and up to the wrist for at least _______. (How long?)
15 seconds
Handwashing procedure: hands are rinsed in what position?
Downward
Handwashing procedure: turn off faucets with ____ to prevent recontamination.
Clean paper towel
What is the handwashing song?
Happy birthday song
The handwashing song should be sung how many times?
Twice
All biological waste, except _____, must be placed in appropriate containers labeled with the _____ symbol.
Urine; biohazard symbol
The accepted biohazard label is colored ______.
Fluorescent orange
Where and how would you discard urine?
Pour urine into a lab sink, avoid splashing and then flush with water.
Disinfection of the sink using a _____ should be performed every _____.
Use 1:5 or 1:10 dilution of sodium hypochlorite, everyday / daily
How is a 1:10 dilution of sodium hypochlorite prepared?
Add 1 part sodium hypochlorite to 9 parts of water
The 1:10 diluted sodium hypochlorite solution is effective for how long?
For 1 month
Describe the biohazard symbol.
Three circles overlapping each other.
Symbol for sharp hazards
Biohazard
Symbol for sharp hazard (if biohazard is not on the choices)
Syringe with diagonal lines
Where do you dispose sharps?
Puncture-resistant containers (red)
The best method of radioactive waste disposal is to store the used radioactive material in a locked, marked room until the background count is down to _____ half-lives for radioiodine.
10
Symbol for chemical hazards pr poisons
Skull with crossed bone
Best first aid if a chemical spilled on your skin
Flush the area with water