Ch.2 Microscopy

Question 1

Microscope

Answer 1

instrument that magnifies an image, enabling very small objects to be observed

Question 2

Resolution

Answer 2

• visual clarity
• the ability to distinguish between two objects
• the greater the resolution, the smaller the distance between two objects

Question 3

Rheostat

Answer 3

adjusts light intensity

Question 4

Base

Answer 4

• supportive bottom piece

- includes substage light

Question 5

Substage light

Answer 5

provides light source

Question 6

Head

Answer 6

• upper part which contains lenses and rotating nosepiece

- ocular lenses, nosepiece, objective lens

Question 7

Ocular lenses

Answer 7

lenses used to observe microscope specimen

Question 8

Nosepiece

Answer 8

rotates to change from one objective lens to another

Question 9

Objective lens

Answer 9

• 4X (scanning)
• 10X (low power)
• 40X (high power)
• 100X (oil immersion)

Question 10

Arm

Answer 10

• connects the head and base

- stage, coarse adjustment knobs, fine adjustment knobs, condenser, iris diaphragm

Question 11

Stage

Answer 11

flat platform where the microscope slide is placed

Question 12

Coarse adjustment knob

Answer 12

raises and lowers the stage in large increments moving it farther or closer from objective lens, controlling focus

Question 13

Fine adjustment knob

Answer 13

used for precision focusing raising or lowering the stage in very small increments

Question 14

Condensor

Answer 14

below the stage, control light intensity

Question 15

Iris diaphragm

Answer 15

beneath condenser, regulates the amount of light passing through the condenser

Question 16

Total magnification (TM)

Answer 16

determined by multiplying the ocular lens power and the objective lens power

Question 17

Working distance

Answer 17

distance between the lens and slide

Question 18

High power

Answer 18

• 40X
• 400X (TM)
• closest lens to slide
• smallest field of view
• largest image

Question 19

Low power

Answer 19

• 10X
• 100X (TM)
• lens medium distance from slide
• intermediate size field of view
• intermediate size image

Question 20

Scanning power

Answer 20

• 4X
• 40X (TM)
• lens farthest from slide
• largest field of view
• smallest image

Question 21

Parfocal

Answer 21

once objectives are switched there is little to no refocusing required

Question 22

Field of view

Answer 22

area of the slide that is visible to you looking though the microscope

Question 23

Depth of field

Answer 23

vertical distance that is in focus