Ch. 3/4/5 lab questions Flashcards
What is an emulsification?
A mixture of two normally non-mixable liquids, such as oil and water.
What is a peptide bond?
A covalent bond between the carboxyl group of one amino acid to the amino group of another amino acid.
What is Benedict’s reagent an indicator for?
glucose
What does Biurent reagent test for?
Proteins
How does Biurent indicate proteins? What do the colors indicate?
It turns from blue to purple.
Purple means proteins.
Blue means negative.
What does Benedict’s need to work? What do the colors indicate?
Benedict’s need heat to work.
Blue means no glucose
Green = some glucose
Yellow = more glucose
Orange = (even) more glucose
Red = a lot of glucose
What does iodine indicate?
Iodine indicates starch.
How does iodine indicate starch?
It will change from yellow-brown to blue-black in the presence of starch.
What is the difference between resolution and magnifiction?
Magnification is how close you can look at something, resolution is how clear you can see it.
List the microscopes in increasing resolution:
-binocular dissecting microscope
-light microscope
-electron microscope
-scanning probe microscope
- binocular dissecting microscope
- light microscope
- electron microscope
- scanning probe microscope
What does it mean if a microscope is parfocal?
The ability of a microscope to stay relatively in focus as the user switches the objectives.
What is the field of view in a microscope?
How much you can see through the objective lens at one time.
What is the relationship between a protein’s shape and the rate of an enzymic reaction?
The rate of reaction changes with the shape of the protein.
What is the optimum point of an enzyme?
The point where it’s moving/working as fast as it can depending on the pH and temperature.
How much enzymic activity does a protein have once its denatured?
It has zero enzymic activity.
