Cellular Pathology Flashcards
What is the order of a test sample
Fixation, cut up room, processing, embedding, cutting, staining
What are the 4 types of microscopy
Brightfield
Polarising
Fluorescence
Electron microscopy
What is brightfield microscopy
The basic microscope set up (light microscopy)
What is polarising microscopy
Polarises are placed on either side of the specimen, perpendicular to each other. This in effect causes a dark field to the observer.
What is fluorescence microscopy
Uses ultraviolet light to excite electrons within molecules in specimens to emit low energy light
What is electron microscopy
Used to get up close and personal with the cells organelles
What is a chromophore
A group of atoms bonded in such a way as absorb light
What is an auxochrome
Ionisable group to allow the chromosphere to bind to tissue
What does the van geison stain
It stains RBC’s yellow and collagen red
What is a trichrome stain
It uses 3 colours used to demonstrate connective tissues such as fibrin, collagen and muscle
What does H&E stain
Nuclei blue and proteins and cytoplasm shades of pink
What does toluidine stain do
Nucleic acids become blue whilst mucins and cartilage becomes purple