Cellular Pathology Flashcards

1
Q

What is the order of a test sample

A

Fixation, cut up room, processing, embedding, cutting, staining

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2
Q

What are the 4 types of microscopy

A

Brightfield
Polarising
Fluorescence
Electron microscopy

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3
Q

What is brightfield microscopy

A

The basic microscope set up (light microscopy)

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4
Q

What is polarising microscopy

A

Polarises are placed on either side of the specimen, perpendicular to each other. This in effect causes a dark field to the observer.

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5
Q

What is fluorescence microscopy

A

Uses ultraviolet light to excite electrons within molecules in specimens to emit low energy light

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6
Q

What is electron microscopy

A

Used to get up close and personal with the cells organelles

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7
Q

What is a chromophore

A

A group of atoms bonded in such a way as absorb light

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8
Q

What is an auxochrome

A

Ionisable group to allow the chromosphere to bind to tissue

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9
Q

What does the van geison stain

A

It stains RBC’s yellow and collagen red

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10
Q

What is a trichrome stain

A

It uses 3 colours used to demonstrate connective tissues such as fibrin, collagen and muscle

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11
Q

What does H&E stain

A

Nuclei blue and proteins and cytoplasm shades of pink

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12
Q

What does toluidine stain do

A

Nucleic acids become blue whilst mucins and cartilage becomes purple

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