Cell Structure + Cell division- biology paper1 Flashcards
What type of cells are bacterial cells?
Prokaryotes
Why are microscopes useful?
Because they allow us to see individual cells and their sub-cellular structures, which we wouldn’t be able to see with the naked eye.
What are the 2 types of microscope?
Light and electron
What do light microscopes do?
They use light and lenses to form an image of a specimen and magnify them. With a light microscope, you can see individual cells and large subcellular structures such as the nucleus.
What do electron microscopes do?
They use electrons to form an image. They have a much higher magnification and resolution than light microscopes. So, we can see much smaller things in detail, like the internal structure of mitochondria and chloroplasts. We can even see tinier things like ribosomes and plasmids.
What is another name for the resolving power?
The resolution
What is the resolution of a light microscope?
around 0.2 μm, or 200 nm. This means that it cannot distinguish two points closer than 200 nm.
What are the 2 types of electron microscope?
The scanning electron microscope (SEM) and The transmission electron microscope (TEM)
What is the highest magnification of a light microscope?
X 2000
What is the highest magnification of an electron microscope?
X 2million
What is the resolution of a scanning electron microscope (SEM)?
10nm
What is the resolution of a transmission electron microscope (TEM)?
0.1nm
What is a field of view?
The area seen when looking through a microscope
The SEM microscope…
has a large field of view so can be used to examine the surface structure of specimens. SEMs are often used at lower magnifications.
The TEM microscope…
is used to examine thin slices or sections of cells or tissues.
What is the magnification of a TEM microscope
X 1 million
How do you prepare a slide using a light microscope?
1) Add a drop of water to the middle of a clean slide
2) Cut up some onion and seperate it out into layers. Use tweezers to peel off some epidermal tissue from the bottom of one of the layers
3) Using tweezers, place the epidermal tissue into the water on the slide
4) Add a drop of iodine solution, Iodine solution is a stain. Stains are used to highlight objects in a cell by adding colour to them.
5) Place a cover slip on top. To do this, stand the cover slip upright on the slide, next to the water droplet. Then carefully tilt and lower it so it covers the specimen.
What is eosin used for?
Making the cytoplasm show up