Cell biology Flashcards
Light microscope use _____ as source of radiation
light
Electron microscope use ____ as source of radiation
electrons
Similarities b/w plant cells and animal cells
-cell membrane
-nucleus—->chromatin—->DNA—->nucleolus
-cytoplasm
-organelles(mitochondria & Golgi)
The organelle that contains in only animal cells but lacks in plant cells is
Centriole-cell division mhr pr
Organelles that contains in only in plants cells
1)cell wall
2)plasmodesmata(plant cellမှာ neighboring cellsတွေကိုlinkလုပ်ပေး)
3)vacuoles (animal cellsမှာပါပေမဲ့ it’s not permanent)
4)Chloroplasts-chlorophyll
In plant cell,vacuole is surrounded by a membrane called____
tonoplast
Which cell is bigger?
plant cell or animal cell?
plant cells
Cell walls are ______ permeable and cell membrane is ______ permeable
cell wall-freely permeable
cell membrane-partially permeable
Image obtained by electron microscope are true-colour.
True or false?
false
they’re false-colour (stainလုပ်ထားတဲ့အပိုင်းတွေကပိုdarkဖြစ်)
Only _____ material is examined by electron microscope.
dead
Glycogen are present in cytoplasm.
True or false?
True
Why electron microscope have better resolution?
b/c electrons has very short wavelength
The _____ membrane of nucleus is continuous with ______
outer
ER
Nucleus is _____ organelle and it is surrounded by ______
Largest organelles
double membrane called nuclear envelope
What are nuclear pores and their function?
nuclear pores are small pores on nuclear envelope
func-exchange of material b/w cytoplasm and nucleus
in nucleus,chromosomes are loosely coiled into _____
chromatin
Function of nucleolus
ribosome synthesis (esp: rRNA)
Chromosome contain ____
DNA
Difference b/w rough ER and Smooth ER
Rough ER contains ribosomes in its membrane and smooth ER lacks ribosome
Function of ribosome
Protein synthesis
Structure of ribosome
large and small subunit
Ribosome is made of _____ and _____
rRNA and protein
rRNA-ribosomal RNA
Functions of smooth ER
synthesis of lipids and steroids. (cholesterol,and reproductive hormones like testosterone and estrogen)
Function of Golgi body
Protein modification and transport
(eg.Golgi vesicles are also used to make lysosomes)
Structure of lysosome
spherical sac surrounded by a single membrane and no internal structure
Lysosome contains_____
digestive (hydrolytic) ensymes
Function of lysosome
-breakdown of macromolecules
-autophagy of organelles
-autolysis pf damaged cells or organelles
-breakdown of unwanted organelles such as old ones or whole cells
eg.mammary glands after lactation
In WBC, lysosomes are used to digest____
bacteria by PHAGOCYTOSIS
phagocytosis-cell eating
Heads of sperm contains____ for ____
acrosome (specialized lysosome)
for digesting path to egg
Cell structure that are surrounded by double membrane
1)nucleus
2)mitochondria
3)Chloroplasts
The inner membrane of mitochondria is folded into _____
cristae
The outer membrane of mitochondria contains ______
transport protein called porin
Membrane permeability of mitochondria
Outer membrane-less selective
Inner membrane-more selective
Which cells contains many mitochondria?
Cell with high demand of energy
(eg.liver and muscle cells)
**RBC do not contains mitochondria **
Function of mitochondria
-ATP synthesis
-lipid synthesis
ATP is _____ molecule sound in all living cells
energy-carrying
Aerobic respiration takes place in _____ of mitochondria
inner membrane and matrix
Mitochondria contains ribosomes.
True or false?
True
The chloroplast and mitochondria contains smaller(70S) ribosomes while cytoplasmic ribosomes is larger (80S).
Bacteria contain 70S ribosomes
Mitochondria and chloroplasts contains DNA.
True or false?
True
It contains small circular DNA like bacteria
(mitochondrial DNA-maternal DNA)
Microtubule structures
long, rigid , hollow tubes found in cytoplasm
(microtubule+actin+intermediate filament—->cytoskeleton)
cytoskeleton maintain cell shape.
Microtubules is made up of _____ protein.
Tubulin- alpha and beta tubulin
(alpha+beta—>dimer)
dimerတွေအများကြီးပေါင်းထား—->protofilaments
Microtubulesကိုcross sectionဖြတ်ရင် 13 protofilamentsရှိ
Centrioles contains _____ of microtubules
nine triplets
Centrioles are found at base of _____
cilia and flagella
acts as microtubule organizing centres
Chloroplasts are surrounded by _____
double membrane
Chloroplasts and mitochondria replicate independently during cell division.
True or false?
True
Main function of chloroplast and shape
photosynthesis
shape-lens-shaped
Mitochondria and chloroplasts contains their own protein synthetic machinery.
True or False?
True
They contains circular DNA and 70S ribosome
Prokaryotic cells lacks ____ and _____contains ____ ribosomes.
Their DNA structure
Lacks nucleus
lacks membrane bound organelles
70S ribosomes
DNA (circular & lies naked in cytoplasm)
Eukaryotic cells contains ____ and _____contains ____ ribosomes.
Their DNA structure
-Nucleus
-membrane bound organelles
-80S ribosomes
-DNA(linear and lied within the nucleus (protein bound DNA)
Source of radiation for light microscope and electron microscope
L- light
E- electron
Lenses that used in light microscope and electron microscope
L-glass
E-electromagnets
Specimen for light microscope and electron microscope
L-living, nonliving or dead
E-nonliving or dead
Stains used for light microscope and electron microscope
L-colored dyes
E-heavy metals
Images of light microscope and electron microscope
L-colored
E-B & W
Organelles with no membrane
1)Nucleolus
2)ribosome
3)centrioles
4)centrosomes
5)microtubules
Organelles with a single membrane
1)RER
2)SER
3)Lysosomes
4)Golgi
SEM shows ______
3D image of surface of specimen
TEM shows______
internal structure of the cell
What is cell fractionation?
separation of cell’s major organelles and other sub-cellular structures
is carried out by CENTRIFUGE
Which type of microscope is used to study
1)internal structure of plant cells and
2)fluorescently labeled nervous tissue?
1)Transmission electron microscope
2)Light microscope
Prokaryotes and Eukaryotes
Prokaryotes
1)Bacteria
2)Archaea
Eukaryotes
1)Animal
2)Plants
3)Fungi
4)Protists (unicellular eukaryotes)
Similarities b/w prokaryotes and eukaryotes
1)cell membrane
2)cytosol
3)DNA
4)Ribosomes
Difference b/w prokaryotes and eukaryotes
1)Location of DNA
Pro-nucleoid (circular DNA)
Eu-Nucleus (linear DNA)
2)size Eu>pro
3)Organelles
Pro-no
Eu-yes
Size of smallest bacteria, typical bacteria and eukaryotic cells
Smallest bacteria: 0.1-1 micrometers
Typical bacteria: 1-5 micrometers
Eukaryotic cells: 10-100 micrometers
All cells have nucleus. True or false?
False
RBC do not have nucleus but skeletal muscle have multinucleus
Function of Nucleus
-stores genetic information
-controls and coordinates the cell
Nucleus is surrounded by _____
double membrane called nuclear envelope
The outer membrane of nuclear envelope contains ____ and inner membrane contain_____
Ribosome
Protein Lamina
Function of Nuclear Lamina
Nuclear lamina aka intermediate filament
Func:maintain the shape of nucleus &
breakdown and assembly of nuclear envelope
Nucleoplasm is_____
cytoplasm inside nucleus and site of metabolic reactions
Chromatin is complex of ____ and _____
DNA and histone protein
Types of chromatin
1)Euchromatin(loose)- centre of the nucleus
2)Heterochromatin(tight)- near inner membrane
Ribosomes are synthesized in _____ but only functions in _____
Nucleus
Cytoplasm
What is 70S ribosome?
ribosome found in prokaryotes (as well as in mitochondria and chloroplasts)
rRNA(30%) + protein(40%)——>70S
What is 80S ribosome?
ribosome contains in eukaryotes
rRNA(60%) + protein (40%)—-> 80S
What are 4 binding site for ribosome?
one for mRNA
and three for tRNA(A,P,E)
Types of ribosome
1)Free ribosomes-cytosol
2)Bound ribosomes-bound to RER and nuclear envelope
3)Polyribosomes
4)70S in mitochondria and chloroplasts
Proteins synthesized from free ribosomes are used ______
mainly within the cell
Proteins synthesized from bound ribosomes are used ______
for secretion and for lysosomes
RER member is continuous with______ and _______
outer membrane of nuclear envelope
SER
ER membrane is physically interconnected.True or False?
True
Golgi membranes are physically interconnected.True or False?
False
Structure of RER
sac-like structures (cristernae)
contains ribosomes on surface
Structure of SER
tubular structure
no ribosomes on surface
Function of RER
1)Protein synthesis
2)Protein Folding
3)Glycosylation( glycoproteinပေါင်း)
4)Packaging (transport vesicles နဲ့ RERဆီပို့)
Why pancreatic cells contains many ribosomes?
b/c the pancreatic cells produces many proteins
Function of SER
1)Glucose metabolism
2)Lipid synthesis____steroid hormones synthesis sites
3)Detoxification of drugs and poisons___liver cells
4)Stores Ca2+__muscle cells
Region of Golgi
1)cis face-receive vesicles from RER and SER products
2)modification and sorting
3)trans face-transport vesicles
Golgi manufactures ______
some polysaccharides
Lysosomes contains
HYDROLYTIC ENZYMES
1)Proteases-protein breakdown
2)Lipases-Lipid breakdown
3)Nucleases-Nucleic acid breakdown
4)Glycosidases-Carbohydrates breakdown
enzymes of lysosomes show greatest performance in ____
acidic environment
Both hydrolytic enzymes and lysosome membranes are synthesized by
RER—>Golgi—>lysosome
Types of vacuoles
1)Food vacuoles
2)Contractile vacuoles
3)Sap vacuole
4)Air vacuole
Function of vacuole
Stores water and mineral
Maintain hydrostatic pressure (turgor)
in plant,central vacuole plays major role in growth of plant cells
In plant,solution inside the central vacuole is called
cell sap