Carbohydrates (Part 3) Flashcards
Hexokinase reagents and products
Reagent: hexokinase and glucose-6-phosphate dehydrogenase
Products: glucose6-phosphate and 6-phosphoglyconate +NADPH + H
A two step reaction that is proposed to be the reference method when a protein-free filtrate is used
Hexokinase
The first step of hexokinase is ________ while the second is ________
specific; non-specific
absorbance in hexokinase is measure at ________
340 nm
A two-step reaction with the first step being specific and the second, non-specific; good method for serum, plasma, urine, and csf; o-dianisidine is the chromogen
Glucose oxidase; “trinder”
Glucose Oxidase “Trinder” reagents
glucose oxidase; peroxidase
Plasma glucose reference interval
-Adults
70-100 mg/dL
Plasma glucose reference interval
-children
70-100 mg/dL
Plasma glucose reference interval
-preemies
25-80 mg/dL
Plasma glucose reference interval
-term babies
60-95 mg/dL
CSF glucose reference interval
60-70% of concommitant plasma levels
Patient preparation instructions for an oral glucose tolerance test (OGTT)
1 minimum of 150 g CHO for 3 days prior to test
2 fasting for 10-16 hrs (water only)
3 discontinue medications that alter glucose levels
4 normal amount of activity before and during test
Three indications for performing an intravenous glucose tolerance test
malabsorption, sprue (Celiac Disease), GI surgery
can be used for preliminary diagnosis of diabetes mellitus; give 75 g oral glucose load or have patient eat a meal; 2 hours later if glucose is _____ may be diabetic
Postprandial Testing; >126 mg/dL
used to diagnose gestational diabetes, performed at _____to_____ weeks gestation; give _____ g glucose load to fasting patient and draw sample at 1 hour; if ____mg/dL perform OGTT
O’Sullivan Test; 24-28 weeks; 50g; >140 mg/dL
