Amino Acid and Protein Analytical Methods

Question 0

reference range for albumin

Answer 0

3.4-5.0 g/dL

Question 1

Reference range for total protein

Answer 1

6.8-8.3 g/dL

Question 2

based on quantitation of nitrogen content of proteins; nitrogen content multiplied by 6.25 to obtain total protein value

Answer 2

Kjeldahl Method

Question 3

in presence of cupric ions, this test yields a violet color; intensity of color produced is proportional to number of peptide bonds present to bind with cupric ions in alkaline solution

Answer 3

Biuret Method

Question 4

based on the refractive index of light; ratio of light refraction in two differing media is related to protein concentration

Answer 4

Refractometry

Question 5

Dye-binding method using bromcresol green or bromcresol purple

Answer 5

Albumin Method

Question 6

quantitative passive diffusion, antigen in serum diffuses from well until antibody excess causes immune precipitation, observed as ring around well

Answer 6

Radial immunodiffusion

Question 7

all antigenic sites are covered with antibody and lattice formation is inhibited; more Ab than Ag

Answer 7

Prozone, Ab excess

Question 8

Optimal proportion of Ab and Ag; 2-3 Ab molecules are present for each Ag molecule; maximum lattice formation and maximum precipitate; insoluble complexes

Answer 8

Equivalence Zone

Question 9

Antigen excess; all Ab sites are saturated by Ag; more Ag than Ab

Answer 9

Postzone

Question 10

the migration of charged solutes or particles (proteins) in a liquid medium under the influence of an applied external electrical field

Answer 10

Electrophoresis

Question 11

three support media for electrophoresis

Answer 11

agarose gel, cellulose acetate, and polyacrylamide gel

Question 12

Acidic pH= protein is a ______

Answer 12

cation

Question 13

the cation migrates to the _______, which is a ____ pole

Answer 13

cathode; negative

Question 14

alkaline pH= protein is an ______, and migrates to the anode which is a _______ pole

Answer 14

anion, positive

Question 15

migration pattern from anode to cathode for serum protein electrophoresis

Answer 15

Albumin, then Alpha, globulins, then Alpha2 globulins, then beta globulins, then Gamma globulins.