C5 - LESSON 2A: STAGES OF B CELL DIFFERENTIATION

Question 1

Antigen Independent Phase

Answer 1

Pro-B Cells

Question 2

Pro-B Cells Important Growth or Transcription factors for differentiation

Answer 2

E2A, EBF, PAX and Interleukin-7

Question 3

Pro-B Cells Distinctive Markers

Answer 3

CD19, CD45R, CD43, CD24 and c-Kit

Question 4

Pro-B Cells Intracellular Proteins

Answer 4

TdT-important in antibody construction
RAG-1 and RAG-2 codes for antibody production

Question 5

Begins at the synthesis of the heavy chain part of antibody

Answer 5

Pre-B Cell

Question 6

Pre-B Cell Markers and proteins lost during process:

Answer 6

CD43, c-Kit and TdT

Question 7

Pre-B Cell Surface Receptors

Answer 7

u chain, surrogate light chain and 2 very short chains
Pre-B cell receptor

Question 8

STAGES OF B CELL DIFFERENTIATION

Question 9

Immature B Cell Distinguishing characteristic

Answer 9

Presence of complete IgM molecule in the cell surface

Question 10

Immature B Cell Other Surface markers

Answer 10

CD21, CD40, MHC Class II molecule

Question 11

Immature B Cell 2 Destinations

Answer 11

Programmed cell death
Cells leave the bone marrow and seed the lymphoid organs

Question 12

Takes place in the spleen

Answer 12

Marginal Zone B cell

Question 13

Lymph nodes and Other SLO

Answer 13

Follicular B Cell

Question 14

Mature B Cell Distinguishing characteristic

Answer 14

Presence of IgM and IgD- same specificity for a particular antigen or group of antigens

Question 15

Mature B Cell

Answer 15

Antigen-dependent phase

Question 16

Antigen-dependent activation

Answer 16

Primary Follicles of Peripheral Lymphoid tissues

Question 17

Identifying Marker

Answer 17

CD25

Question 18

found on both activated T and B cells

Answer 18

CD25

Question 19

acts as a receptor for interleukin-2 (IL-2)

Answer 19

CD25

Question 20

• a growth factor produced by T cells

Answer 20

interleukin-2 (IL-2)

Question 21

Spherical or ellipsoidal

Answer 21

Plasma Cells

Question 22

Plasma Cells Characterized by

Answer 22

Abundant cytoplasmic Immunoglobulin
Nucleus: eccentric or oval (heavily clamped chromatin-stains dark)
Abundant ER and well-defined golgi zone

Question 23

Present in the germinal center of the peripheral lymphoid organ

Answer 23

Plasma Cells

Question 24

Found in germinal layer

Answer 24

Memory Cells

Question 25

Has longer life span

Answer 25

Memory Cells

Question 26

Represents progeny of antigen stimulated B cells

Answer 26

Memory Cells

Question 27

Similar in appearance with unstimulated B cells

Answer 27

Memory Cells