C. perfringens

Question 1

Mention sime characters of C. perfringens according to: 
Gram stain
capsule
spore shape 
air state
hemolysis on blood agar 
types

Answer 1

psitive large bacilli
capsulated
oval, subterminal, non projecting 
anaerobics
complete hemolysis 
A, B, C, D, E

Question 2

Give some of the virulance factors of C. perfringens

Answer 2

1_Alpha toxin«lecithinase»
2_Theta toxin
3_DNAase,hyaluronidase,collagenase
4_Enterotoxin

Question 3

what is the I. D for C. per

Answer 3

more than 108 vegetative from

Question 4

what’s the cause and symptoms for food poisining caused by C. perfringens??

Answer 4

Enterotoxins released in the gut by spores after ingestion of vegitative form on reheated meat.

diarrhea, no fever, no vomiting

Question 5

Mention the categories of tissue destroying Clostridia.

and what’s the role of each one?

Answer 5

1_sacchrolyric organisms
C.perfringens
C.oedematines
C.septicum
fermentation and gas production

2_Proteolytic organisms
C. histolyticum
C. sporogenes
black colour and foul odor develop

Question 6

How does myonecrosis occurs in C. perfringens

and what’s the causes favour the germination of spores?

Answer 6

By contamination of wounds with soil containing the spores

1_deep lacerated wound,car accedent,war wounds
2_deep stabbing wound
3_The presence of forign bodied
4_mixed infection with aerobic pyogenic bac.
5_decreased blood supply

Question 7

Mention the steps of pathogenisis of C. perfringens

Answer 7

1_contamination
2_Germination
3_sacchrolytic Clostridia ferment sugar,distend tissue,interfere with B.supply,hypoxia,death
4_Necrotizing toxins,collagenase,hyal ase, spread the infection
5_Proteolytic organisms digest dead tissue leading to black color and foul odor of the wound.

Question 8

what’s the look of the wound and symptoms accompanied with myonecrosis?

Answer 8

oedematous, foul odor, dark in color, with crepitations in adjacent tissue.

accompanied with toxaemia, shock, multi organ failure, death.