Brucella

Question 1

aerobic? capsulated? motile? intracellular?

Answer 1

strict aerobes, non capsulated, non motile, facultative intrac

Question 2

Mention the species of Brucella

Answer 2

B. abortus
B. suis
B. melitenisis
B. canis

Question 3

culture media characters?

Answer 3

fastedious
slow growth
required enriched media:
brucella agar, brain heart infusion, trypticase soya agar, choclate agar.

Question 4

regarding to 
oxidase, urease, catalase 
H2s
co2 
sugar fermentation 
dominant AG

Answer 4

all positive 
hs by abortus, suis 
co2 needed by abortus 
all non suger fermenter
abortus: A
melitenis: M
suis: A, M
B. melitenisis is not inhibitied by basic fuchsine, thionine, pyronine

Question 5

mention diseases caused by Brucella species?

Answer 5

B. abortus, B. canis make mild supparative febrile inf
B. suis, make severe supparative inf may lead to destroy lymphoreticular organs and kidney
Br. melitenisis cause most sever prolonged recurring diseases.

Question 6

how udulant fever can transmit?

Answer 6

through mucous mem of the oropharynx by: 
inhalation 
ingesion 
skin abrasion 
throug conjunctiva

Question 7

mention some symptoms for brucellosis

Answer 7

intermittent fever.
weakness, malaise, sweating, headache, joint and muscle pain.
enlarged lymph nodes, liver, spleen
3%fetal due to endocarditis

Question 8

How can you diagnose brucellosis.

Answer 8

1-acute: blood culture on duplicate agars: white glisting colonies.

2-chronic: serology; standard tube agglutination test. 
rapid slide agglutination test. 
coombs antiglobulin test. 
ELISA
3_PCR
4_brucillin test 
5_biobsy

Question 9

why STAT can be false negative? how to over come it?

Answer 9

prozone phenomena
IgA blocking AB

by using rapid slide agglutination test

Question 10

Treatment?

Answer 10

rifampicin +Streptomycin or tetracycline