Burkholderia Flashcards
Burkholderia is a gram ______ bacteria.
Negative
(rods)
Burkholderia is catalase ______.
Positive
True/False. Burkholdia is aerobic.
True
Which species of Burkholderia causes glanders in horses?
B. mallei
What species of Burkholderia causes melioidosis in sheep and goats?
B. pseudomallei
Which species of Burkholeria is frequently found in cystic fibrosis patients (humans)?
B. cepacia
Which species of Burkholderia was bioweaponized in WWI?
B. mallei
Biosafety level 3 agent
True/False. Glanders is a reportable disease.
TRUE. REPORT IT.
What regions is glanders endemic?
Now mainly Asia and South Africa
How is B. mallei transmitted?
Ingestion of contaminated feed or water
Inhalation
Wounds
What symptoms woud you expect to see with glanders disease?
pyogranulomatous nodules, and ulcers
Orchitis - inflammation of testes
Respiratory tract infection
[nodular lesions in lymphnodes, lung, liver, spleen, nose and skin]
What is the reservoir host in endemic regions for B. pseudomallei?
Rodents in tropical and subtropical regions.
(amoebas are reservior hosts too - endosymbiont of environmental amoeba)
Since B. pseudomallei is a real saprophyte, what kind of enivronment would you expect to find it in?
Water rich environments
Swampy terrian
Rice paddies
What is meliodosis?
A pyogranulomatous infection that is similar to glanders disease
What disease would you see in a horse infected with B. pseudomallei?
Pseudoglanders
[mimcics glanders, less contagious, less severe, and is not reportable]
What would you expect to see in a dog infected with B. pseudomallei?
Febrile diseases with localizing suppurative foci
(very important in military dogs during war in Vietnam - very tropical region)
What symptoms would you expect to see in a sheep infected with B. pseudomallei?
Arthristis and lympandentitis
What symptoms do you expect to see in goats and swine infected with B. pseudomallei?
More of a chronic condition
Goats and swine: loss of condition, respiratory and CNS disturbances, arthritis and mastitis
Swine: Abortion and diarrhea
What is the official test for glanders in horses? What is another specific test for glanders you can perform if you get a false positive?
Complement fixation test - beware of false positives
Mallein test (intradermo test) - type IV hypersensitivity, cellular
How would you treat a horse infected with glanders?
First of all, report that shit.
Then cull all those horses.
[no vx]
How would you treat a horse infected with meliodosis?
Drainage of abscesses and antimicrobials (susceptibility profile)
[no vx]
Which species grows faster? B. pseudomallei or B. mallei?
B. pseudomallei <48 hrs
Which species of Burkholderia grows on 2% NaCl?
B. pseudomallei
Which species of Burkholderia oxidizes lactose?
B. pseudomallei
Which species of Burkholderia is motile?
B. psuedomallei
Which selective media can you use to isolate B. pseudomallei in mixed infections?
Ashdown’s selective medium
