Biomolecule Flashcards

1
Q

Commercially glucose is prepared by

A

Hydrolysis of starch in presence of dil H2SO4 under pressure and temp of 393K

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2
Q

The presence of 6 c atoms in glucose was confirmed by

A

Reaction with HI

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3
Q

How was the presence of carbonyl grp confirmed in glucose

A

Reaction with hydroxyl amine and hydrogen Cyanide it formed oxime and cyanohydrin

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4
Q

How was the presence of aldehyde grpmconfirmed in glucose

A

Rn with mild oxidizing agent like Br water yield gluconic acid

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5
Q

The presence of 5 OH grp was confirmed by

A

Rn with Acetic anhydride

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6
Q

The presence of 1° alcoholic grp was confirmed by

A

Oxidation of glucose and gluconic acid in presence of nitric acid yield saccharic acid

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7
Q

In carbohydrates D and L configuration is with resp to

A

Glyceraldehyde

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8
Q

Despite having aldehyde grp which of the following is not given by aldehydes

A

Schiffs test and reaction with hydrogen sulphite addition compound, pentacetate of glucose does not react with hydroxylamine

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9
Q

How is Alpha form of glucose obtained by

A

Crystallization from concentrated solution of glucose at 303K

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10
Q

How is beta form of glucose obtained

A

Crystallization from hot and saturated aq solution at 371K

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11
Q

Which has high melting point beta glucose or alpha glucose

A

Beta glucose

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12
Q

What is glycosididc linkage

A

Linkage btw two Monosacchrides through oxygen atom after the loss of water

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13
Q

In Sucrose Glycosidic linkage is btw

A

C1 of alpha (D) glucose and C2 of beta ( D) fructose

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14
Q

In maltose glycosidic linkage is btw

A

C1 of one glucose and C4 of another glucose ( Both are alpha D glucose)

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15
Q

In Lactose glycosidic linkage is btw

A

C1 of beta ( D) galactose and C4 of beta ( D) glucose

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16
Q

Starch is a polymer of

A

Alpha glucose

17
Q

In amylose the linkage is

A

C1 and C4

18
Q

In amylopectin the linkage is

A

C1 and C4 for unbranched and C1 and C6 for branched

19
Q

Cellulose is made up of

A

Beta D glucose

20
Q

Why is glycogen known as animal Starch?

A

The structure of glycogen is similar to Starch but glycogen is more branched

21
Q

Hydrolysis of proteins obtain

A

Alpha Amino acids

22
Q

A: glycine has sweet taste
R: tyrosine was first obtained from cheese

A

Both A and R are true but they are not related to each other

23
Q

Essential amino acids

A

PVT TIM HALL
Phenylphalanine , valine , Tryptophan, threonine , isoleucine , Methionine, histidine, Arginine, lucine , Lysine

24
Q

Amino acids bhave like

A

Salts

25
Q

Most naturally occurring Amino acids have

A

L form

26
Q

What is Peptide linkage ?

A

Peptide linkage is an amide linkage formed by cooh and nh2 grp of Amino acids

27
Q

Insulin contains

A

51 Amino acids

28
Q

Fibrous proteins are

A

Insoluble in water ( eg keratin and myosin )

29
Q

Globular proteins are

A

Soluble in water

30
Q

What is denaturation ?

A

A protein in its unique 3 dimensional geometry and biological activity is called a native protein . When subjected to a change in temp and pH the hydrogen bonds are disturbed . Thus the globules unfold and the helix get uncoiled and protein loses its biological activity

31
Q

Almost all enzymes are

A

Globular proteins

32
Q

The b vitamin which can be stored in our body is

A

Vit B12