Bacteriology 6: Staphylococci Flashcards

1
Q

What are bacteria groups based on?

A

The type of inflammation they induce

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2
Q

What type of inflammation do pyogenic bacteria form?

A
  • Puss forming
  • Acute inflammation
  • See predominantly neutrophils
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3
Q

What type of inflammation do pyogranulomatous bacteria form?

A

Mixture of acute and chronic inflammation
- We will see a mixture of cells
–Polymorphonuclear neutrophils
– Macrophages
– Lymphocytes
– + or - eosinophils if fungal

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4
Q

What type of inflammation do Granulomatous bacteria form?

A

We see central necrosis surrounded by:
- giant epitheliod cells (activated macrophages)
- macrophages
- lymphocytes
- Granulocytes
- Thick fibrous capsule

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5
Q

What type of inflammation do necrotizing bacteria form?

A

We see a lot of cellular debris:
- Toxic cells
- Inflammatory cells
- the causative bacteria

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6
Q

What are the types of Gram + cocci bacteria we are discussing?

A
  • Staphylococci
  • Streptococci
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7
Q

What are types of pyogenic bacteria?

A
  • Staphylococci (gm+ cocci)
  • Streptococci (gm+ cocci)
  • Trueperella (gm+ rods)
  • Corynebacterium (gm+ rods)
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8
Q

What are the types of gram + rods?

A

Clostridium
Actinomyces
Nocardia
Dermatophilus
Rhodococcus
Trueperella
Corynebacterium
Mycobacteria

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9
Q

What are types of pyogranulomatous bacteria?

A
  • Staphylococci (gm+ cocci)
  • Actinomyces (gm+ rods)
  • Nocardia (gm+ rods)
  • Dermatophilus (gm+ rods)
  • Rhodococcus (gm+ rods)
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10
Q

What are types of granulomatous bacteria?

A

Mycobacteria (gm+ rods)

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11
Q

What are types of necrotizing bacteria?

A

Clostridium (gm+ rods)

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12
Q

Where do pyogenic bacteria cause dx?

A

Extracellular

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13
Q

What are hallmarks of inflammation?

A
  • Increased number of cells in fluid
  • Increased number of protein in fluid
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14
Q

What is coagulase test meaning?

A
  • It differentiates between weakly virulent and highly virulent
    + mean highly virulent
  • mean weakly virulent
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15
Q

Where are coagulase positive staphylococci found?

A

Skin mucocutaneous junctions
Distal nasal passage
External nares
Rectum

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16
Q

Where are coagulase negative staphylococci found?

A

skin- normal flora

17
Q

Factors helping staphylococci be good pathogens

A

Capsule or pseudocapsule
Exotoxins- Leukotoxins and hemolysins
Intracellular survival
Biofilm survival

18
Q

How does the capsule help cause disease?

A
  • Anti-phagocytic
19
Q

Non-pathogenic (coag-neg) staphylococci interaction with macrophages?

A

Macrophages can easily phagocytize coag-neg staphylococci

20
Q

How do exotoxins help cause disease?

A

Many different exotoxin
Hemolysins and leukotoxins are important for virulence
—- These are cytotoxic and lethal (aka damage cells)
—- Leukotoxins kills phagocytes therefore assist in evading the immune system

21
Q

How does being a facultative intracellular bacteria assist in causing disease

A

Evade the immune system by:
- Killing phagocytes
- avoid antibodies
- avoiding abx that act extracellular

22
Q

What kind of infection can facultative intercellular bacteria cause?

A

PI - chronic

23
Q

What kind of pathogen are staphylococci?

A

Opportunistic pathogens: require compromise for host-pathogen interaction

24
Q

Are any staphylococci pathognomic?

A

No

25
Q

diseases caused by staphylococci?

A

Pyoderma (VERY common)
Mastitis
Greasy pig disease (Exudate dermatitis)
Musculoskeletal infections: Osteomyelitis, Septic arthritis
Urogenital tract infections: Cystitis, Prostatitis

26
Q

What rules are you supposed to follow in diagnosing staphylococci?

A

Part of normal flora in sterile site so follow four point rule:
- the sample must be collected in appropriate fashion from site of infection
- there must be evidence of inflammation in the sample
- good if there is evidence of bacteria in the sample
- the bacteria isolate must have the opportunity and be capable of causing this dx