Bacterial part I

Question 1

Phase of bacterial growth metabolically active

Not yet dividing

Answer 1

Lag phase

Question 2

Phase of bacterial growth wherein cell division is rapid

Increase population of MO

Answer 2

Log phase or exponential phase

Question 3

Phase of bacterial growth wherein rate of division = death rate

Answer 3

Stationary phase

Question 4

Phase of bacterial growth where nutrients are greatly reduced, toxins increased

Answer 4

Death phase

Question 5

Bactericidal antibiotics act in this phase

Answer 5

Log phase or exponential phase

Question 6

Cell division of bacteria

Answer 6

Binary fission

Question 7

Small DNA molecule found in microorganisms that are separated from their chromosomal DNA

Answer 7

Plasmid

Question 8

Process of transferring plasmids from one microbe to another

Answer 8

Horizontal gene transfer or bacterial recombination

Question 9

Types of bacterial recombination (3)

Answer 9

Conjugation
Transformation
Transduction

Question 10

Type of bacterial recombination for direct contact and used sex pili

Answer 10

Conjugation

Question 11

Type of bacterial recombination where dna is left in envt then engulfed by recipient bacteria

Answer 11

Transformation

Question 12

Function of pili

Answer 12

Attachment

Question 13

Type of bacterial recombination used bacteriophage or bacterial virus to transfer plasmids

Answer 13

Transduction

Question 14

4 growth phase

Answer 14

Lag
Log/exponential
Stationary
Death phase

Question 15

Phage that kills bacteria immediately

Answer 15

Virulent phage

Question 16

Phage that combines with bacteria then kills after

Answer 16

Temperate phage

Question 17

Basic component of bacterial cell wall

Answer 17

Glycoprotein

Question 18

Other term for peptidoglycan

Answer 18

Murein layer

Question 19

Characteristic of gram + cell wall

Answer 19

Thicker cell wall
Thick peptidoglycan layer
Teichoic acid (attached to pep)

Question 20

Characteristic of gram - cell wall

Answer 20

Thin cell wall
Thin peptidoglycan
Lipopolysaccharide - activated immune response

Question 21

Toxin of gram +

Answer 21

Exotoxin (teichoic acid)

Question 22

Toxin of gram -

Answer 22

Endotoxin (lipopolysaccharide)

Question 23

Acid fast cell wall characteristic

Answer 23

Waxy substance with Mycolic acid
Resistant to gram staining
Resistant to decolorization

Question 24

Gram + bacteria produces endotoxin and exotoxin

Answer 24

Listeria monocytogenes

Question 25

Gram staining procedure

Answer 25

VIAS!!

Crystal violet
Iodine (violet)
Alcohol (decolorization)
Safranin/carbolfuchsin (red)

Question 26

Acid fast staining procedure

Answer 26

CAM!

Carbolfuchin (red)
Acid - alcohol
Methylene blue (blue)

Question 27

Gram staining procedure

Gram + color:_____
Gram -color:_______

Answer 27

Violet

Red

Question 28

Acid fast staining aka

Answer 28

Ziehl-neelsen stain

Question 29

In acid fast staining
Gram + color: ______
Gram -:______
Acid fast:______

Answer 29

Blue to violet
Blue
Red