Bacterial cell division

Question 1

What is binary fission

Answer 1

1 cell spilts into 2 cells
bacterial reproduction/cell division
archaeal

Question 2

Correct order for binary fission

Answer 2

The cell elongates,
replicates its chromosome,
separates the chromosome into two parts of the cell,
a septum forms at midcell.
cell separated

Question 3

Bacteria reproductive strategies include all of the following EXCEPT
A) binary fission
B) budding
C) multiple fission
D) formation of multinucleoid filaments that break apart into uninucleate spores
E) fusion of two bacterial cells that then split into four uninucleate cells

Answer 3

E) fusion of two bacterial cells that then split into four uninucleate cells

Question 4

What happens during the cell division process of binary fission

Answer 4

signal for binary fission
DNA replicates
septum forms
chromosomes segregate
peptidoglycan synthesis

Question 5

Cell division binary

Answer 5

Prokaryotes and Eukaryotes
replicate DNA
segregate DNA into daughter cells
Division of cellular components
Components include ribosome subunits, enzymes, and transport proteins 
see page 3 on power point

Question 6

What is bacterial growth

Answer 6

increase in cell NUMBER not size

Question 7

what is binary fission

Answer 7

1 bacteria cell splits into 2 cells (each is generation)

Question 8

The ________ ________ is the length of time it takes for a population of microorganisms to double in number.

Answer 8

generation time

Question 9

Time it takes for one round of binary fission to occur

Answer 9

generation time

Question 10

steps of binary fission

Answer 10

cell elongation
DNA replication
septum formation
complete of septum with walls
cell separates

Question 11

What signals binary fission

Answer 11

nutrient concentration

environmental conditions

Question 12

check what nutrient concentrations or environmental conditions signal binary fission

Answer 12

nutrient-rich environment

pH neutral

Question 13

What is the septum formed from

Answer 13

FtsZ ring

Question 14

binary fission Chromosome replication start

Answer 14

•origin of replication in DNA

Question 15

Chromosome replication initiated by

Answer 15

DnaA binding (initiation protein)

Question 16

Which way do chromosomes replicate

Answer 16

bidirectional

Question 17

When does chromosome replication stop

Answer 17

-Until reaches terminus

•Directly opposite origin

Question 18

Complex containing enzymes necessary for DNA replication

Answer 18

replisome

Question 19

•Chromosomes separate as DNA replication occurs T/F

Answer 19

true

Question 20

Chromosome replication and partitioning happen concurrently (at the same time) T/F

Answer 20

True

see figure on slide called chromosome replication

Question 21

The \_\_\_\_\_\_\_\_ is a group of proteins needed for DNA synthesis that assembles at the origin of replication.
A) septum
B) replisome
C) prospore
D) None of the choices are correct.

Answer 21

replisome

Question 22

The site at which replication of DNA starts in bacterial cells is known as the \_\_\_\_\_\_\_\_.
A) origin of replication
B) initiation center
C) replication initiation site
D) start complex

Answer 22

a) origin of replication

Question 23

•Genome replication time is independent or dependent of generation time (E Coli)

Answer 23

independent==means they will work separate

Question 24

septum formation

Answer 24

inward growth of cytoplasmic membrane and cell wall

before cell wall separates into two cell

Question 25

Site of septum formation minCDE

Answer 25

Limits z ring formation | oscillate between cell poles

Question 26

``` During cytokinesis, a critical step in septation is the assembly of the ________. A) replisome B) spindle body C) Z ring D) cytoskeletal spiral ```

Answer 26

C) Z ring

Question 27

Only place Z ring forms

Answer 27

mid cell, where there is no minCDE

Question 28

What is the MinCDE?

Answer 28

prevent the formation of the Z-ring at poles by oscillating from pole to pole leaves middle open

Question 29

What does level high minC at poles do

Answer 29

blocks septum formation

Question 30

What does minD do

Answer 30

forms filaments and interacts with plasma membrane

Question 31

what does minE do

Answer 31

controls min D oscillation, directs FtsZ ring formation divisome complex

Question 32

Why does Z ring form at mid cell only.

Answer 32

No min CDE

Question 33

What happens with nucleoid occlusion for septum formation

Answer 33

prevents premature septum formation | Z ring forms after most daughter chromosomes separate

Question 34

T/F most daughter chromosomes form before z ring forms

Answer 34

True

Question 35

The site of septum formation in E. coli is determined by the MinCDE proteins. Which of the following statements regarding the positioning of the septum are correct?

Answer 35

The concentration of MinCDE proteins is lowest in the midpoint of the cell.

Question 36

Which of the following statements regarding FtsZ are correct?

Answer 36

- FtsZ localization determines the site of cell wall growth by recruiting PBPs for peptidoglycan synthesis. - FtsZ polymerizes to form the Z ring that determines the site of septum formation. see slide 7

Question 37

MinCDE forms Z ring that determines

Answer 37

site for septum formation

Question 38

Divisome

Answer 38

The division of bacterial cell begins

Question 39

What is the divisome?

Answer 39

division apparatus in the cell formed by Fts proteins

Question 40

4 steps of septum formation

Answer 40

site for septum formation assemble z ring form cell division apparatus/machine (divisome) Constriction of z-ring, septum forms

Question 41

FtsZ

Answer 41

forms ring around center of cell | peptidoglycan synthesis for septum growth

Question 42

FtsA ZipA

Answer 42

Anchor Z ring to plasma membrane

Question 43

FtsK

Answer 43

Chromosome segregation and separation of chromosome dimers

Question 44

FtsEX

Answer 44

Coordinate hydrolases for septum growth and cell separation

Question 45

FtsW/FtsI

Answer 45

peptidoglycan synthesis in septum

Question 46

FtsN, FtsQLB

Answer 46

Coordinate ring constriction with septum growth

Question 47

What happens when FtsZ breaks down

Answer 47

cell division occurs

Question 48

FtsZ functions like and is related to

Answer 48

polymerizes to form filaments depolymerizes to constrict cell Hydrolyze GTP Highly conserved

Question 49

What is FtsZ related to?

Answer 49

tubulin

Question 50

Provides energy for polymerization/depolymerization (assembly/disassembly) of FtsZ ring

Answer 50

Hydrolyzes GTP

Question 51

Highly conserved FtsZ

Answer 51

-Found in almost all eubacteria, most archaea, and organelles of eukaryotes

Question 52

Microbial ________ is an increase in cell constituents that may or may not be accompanied by an increase in cell number.

Answer 52

growth