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Cellbio > Bacterial cell division > Flashcards

Bacterial cell division Flashcards

1
Q

What is binary fission

A

1 cell spilts into 2 cells
bacterial reproduction/cell division
archaeal

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2
Q

Correct order for binary fission

A 
The cell elongates,
replicates its chromosome,
separates the chromosome into two parts of the cell,
a septum forms at midcell.
cell separated
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3
Q

Bacteria reproductive strategies include all of the following EXCEPT
A) binary fission
B) budding
C) multiple fission
D) formation of multinucleoid filaments that break apart into uninucleate spores
E) fusion of two bacterial cells that then split into four uninucleate cells

A

E) fusion of two bacterial cells that then split into four uninucleate cells

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4
Q

What happens during the cell division process of binary fission

A 
signal for binary fission
DNA replicates
septum forms
chromosomes segregate
peptidoglycan synthesis
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5
Q

Cell division binary

A 
Prokaryotes and Eukaryotes
replicate DNA
segregate DNA into daughter cells
Division of cellular components
Components include ribosome subunits, enzymes, and transport proteins 
see page 3 on power point
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6
Q

What is bacterial growth

A

increase in cell NUMBER not size

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7
Q

what is binary fission

A

1 bacteria cell splits into 2 cells (each is generation)

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8
Q

The ________ ________ is the length of time it takes for a population of microorganisms to double in number.

A

generation time

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9
Q

Time it takes for one round of binary fission to occur

A

generation time

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10
Q

steps of binary fission

A 
cell elongation
DNA replication
septum formation
complete of septum with walls
cell separates
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11
Q

What signals binary fission

A

nutrient concentration

environmental conditions

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12
Q

check what nutrient concentrations or environmental conditions signal binary fission

A

nutrient-rich environment

pH neutral

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13
Q

What is the septum formed from

A

FtsZ ring

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14
Q

binary fission Chromosome replication start

A

•origin of replication in DNA

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15
Q

Chromosome replication initiated by

A

DnaA binding (initiation protein)

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16
Q

Which way do chromosomes replicate

A

bidirectional

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17
Q

When does chromosome replication stop

A

-Until reaches terminus

•Directly opposite origin

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18
Q

Complex containing enzymes necessary for DNA replication

A

replisome

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19
Q

•Chromosomes separate as DNA replication occurs T/F

A

true

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20
Q

Chromosome replication and partitioning happen concurrently (at the same time) T/F

A

True

see figure on slide called chromosome replication

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21
Q 
The \_\_\_\_\_\_\_\_ is a group of proteins needed for DNA synthesis that assembles at the origin of replication.
A) septum
B) replisome
C) prospore
D) None of the choices are correct.
A

replisome

22
Q 
The site at which replication of DNA starts in bacterial cells is known as the \_\_\_\_\_\_\_\_.
A) origin of replication
B) initiation center
C) replication initiation site
D) start complex
A

a) origin of replication

23
Q

•Genome replication time is independent or dependent of generation time (E Coli)

A

independent==means they will work separate

24
Q

septum formation

A

inward growth of cytoplasmic membrane and cell wall

before cell wall separates into two cell

25
Q

Site of septum formation minCDE

A

Limits z ring formation

oscillate between cell poles

26
Q 
During cytokinesis, a critical step in septation is the assembly of the \_\_\_\_\_\_\_\_.
A) replisome
B) spindle body
C) Z ring
D) cytoskeletal spiral
A

C) Z ring

27
Q

Only place Z ring forms

A

mid cell, where there is no minCDE

28
Q

What is the MinCDE?

A

prevent the formation of the Z-ring at poles by oscillating from pole to pole
leaves middle open

29
Q

What does level high minC at poles do

A

blocks septum formation

30
Q

What does minD do

A

forms filaments and interacts with plasma membrane

31
Q

what does minE do

A

controls min D oscillation,
directs FtsZ ring formation
divisome complex

32
Q

Why does Z ring form at mid cell only.

A

No min CDE

33
Q

What happens with nucleoid occlusion for septum formation

A

prevents premature septum formation

Z ring forms after most daughter chromosomes separate

34
Q

T/F most daughter chromosomes form before z ring forms

A

True

35
Q

The site of septum formation in E. coli is determined by the MinCDE proteins. Which of the following statements regarding the positioning of the septum are correct?

A

The concentration of MinCDE proteins is lowest in the midpoint of the cell.

36
Q

Which of the following statements regarding FtsZ are correct?

A
  • FtsZ localization determines the site of cell wall growth by recruiting PBPs for peptidoglycan synthesis.
  • FtsZ polymerizes to form the Z ring that determines the site of septum formation.

see slide 7

37
Q

MinCDE forms Z ring that determines

A

site for septum formation

38
Q

Divisome

A

The division of bacterial cell begins

39
Q

What is the divisome?

A

division apparatus in the cell formed by Fts proteins

40
Q

4 steps of septum formation

A

site for septum formation
assemble z ring
form cell division apparatus/machine (divisome)
Constriction of z-ring, septum forms

41
Q

FtsZ

A

forms ring around center of cell

peptidoglycan synthesis for septum growth

42
Q

FtsA ZipA

A

Anchor Z ring to plasma membrane

43
Q

FtsK

A

Chromosome segregation and separation of chromosome dimers

44
Q

FtsEX

A

Coordinate hydrolases for septum growth and cell separation

45
Q

FtsW/FtsI

A

peptidoglycan synthesis in septum

46
Q

FtsN, FtsQLB

A

Coordinate ring constriction with septum growth

47
Q

What happens when FtsZ breaks down

A

cell division occurs

48
Q

FtsZ functions like and is related to

A

polymerizes to form filaments
depolymerizes to constrict cell
Hydrolyze GTP
Highly conserved

49
Q

What is FtsZ related to?

A

tubulin

50
Q

Provides energy for polymerization/depolymerization (assembly/disassembly) of FtsZ ring

A

Hydrolyzes GTP

51
Q

Highly conserved FtsZ

A

-Found in almost all eubacteria, most archaea, and organelles of eukaryotes

52
Q

Microbial ________ is an increase in cell constituents that may or may not be accompanied by an increase in cell number.

A

growth

Cellbio (17 decks)

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