BACTE LAB LA9 Flashcards
Cultures for Cytomegalovirus must always be frozen at less than or equal to ________________________ when sent to a referral lab.
-70 °C (dry ice)
Enumerate the Collection Criteria
- From the actual site of infection.
- Collect the specimen at optimal times.
- Collect specimens prior to administration of antimicrobial agents.
- Sufficient quantity
- Properly labeled.
- Ensure that the proper transport system is utilized for orders with more than one test.
- Minimize transport time
Enumerate the Rejection Criteria
- Clerical Error
- Inappropriate Containers/ Conditions
- Delay in Transit or Improper Transport
- Inappropriate specimens
- Inadequate specimens
- Excess specimens
Information on the requisition does not match the information on the specimen label.
Clerical Error
If the patient name or source does not match, the specimen
should be collected again
Clerical Error
There is no patient identification on the specimen container
Clerical Error
The specimen is not submitted in the appropriate transport container
Inappropriate Containers/ Conditions
The container is leaking.
The container is grossly contaminated externally.
Inappropriate Containers/ Conditions
The specimen transport time is more than 2 hours.
Unpreserved specimen.
Delay in Transit or Improper Transport
The specimen is received in a fixative such as formalin will not be accepted unless these are stools for ____________________
Ova & Parasite examinations
The specimen is dried up.
Inappropriate specimens
used to check viability of SPUTUM Samples
Suitability Checking
Gram stain of expectorated sputum reveals fewer than _____________ and
more than __________________ per LPF an mixed bacterial biota are rejected
25 WBCs, 10 epithelial cells
An anaerobic culture is requested on a specimen which anaerobes re
indigenous.
Inappropriate specimens
The specimen is inadequate (low volume) to perform all tests
requested.
One swab was submitted for multiple requests for various organisms
Inadequate specimens
has submitted than one specimen from the same source and from
the same patient on the same day; blood cultures are exception.
Excess Specimens
- Sputum
- Endotracheal Aspirate
- Bronchial Lavage
- Throat Swab
- Nasopharyngeal Swab
RESPIRATORY SAMPLES
Plates used for Respiratory Samples
BAP-CAP-GBA-MAC-THIO
THIO meaning
Thioglycollate Broth
- CSF
- Pleural, Pericardial, Peritoneal Fluid
BODY FLUIDS
Plates used for Body Fluids
BAP-CAP-GBA-MAC-TSB
TSB meaning
Tryptic Soy Broth
Plates used for Blood
BAP-CAP-GBA-MAC
GBA meaning
Gentamicin Blood Agar
GBA is for the growth of __________________
Streptococcus Pneumoniae
- Cervicovaginal Discharge
- Penile Discharge
Genital Specimen
Plates used for Genital Specimens
CAP- MTM
MTM meaning
Modified Thayer-Martin
MTM is for the growth of _______________
Neisseria gonorrheae
- rectal swabs
- stool
Stool Specimen
Plates used for Stool Specimens
MAC(SMAC)- SSA- TCBS- APW- SEL F
SMAC meaning
Sorbitol MacConkey
SMAC is for the growth of _______________
Escherichia coli
SSA Meaning
Salmonella- Shigella Agar
TCBS Meaning
Thiosulfate-Citrate-Bile Salt-Sucrose agar
TCBS is for the growth of _______________
vibrio spp.
APW meaning
Alkaline peptone water
APW is for the growth of ________________________
vibrio spp.
SEL F meaning
Selenite F- Salmonella (Broth)
plates for Urine Sample
BAP- MAC
- Wound swab/ wound discharge
- Abscess
- Ear discharge
Exudate
INCUBATION FOR 19-24 Hours Anaerobically
BAP and CAP in Respiratory Samples
CAP and MTM of Genital Specimen
INCUBATION FOR anaerobes utilizes _____________
candle jar or CO2 incubator
INCUBATION for Aerobes
- All of MAC
- BAP for urine and exudates
- Plates for Stool Sample
- Broth
