B3.050 - Prework Pre-Transfusion Workup Flashcards
What is the endpoint of al serological tests
agglutination
what are the 2 steps of serological tests
sensitization agglutination
what is sensitization
Abs binding to RBCs surface Ag
what is agglutination
sensitized RBCs are bridged together to form the lattice
what is the AGT
antiglobulin test used to enhance agglutination usually require for clinical significant Ab detention
Type of RBC antibodies
IgM, IgG
Which is bigger IgM or IgG
IgM
what are cold antibodies
IgM - react best at 22 in IS phase
what do IgM antibodies do
Fix complement and cause intravascular hemolysis
what do IgG do and are they warm or cold
warm - react at 37 in AHG phase
what is the IS phase in a tube test
immediate spin phase When you put pt serum in a tube with a commercial reagent RBCs
describe the steps of phase 1 tube test
- mix 2. centrifuge @ RT 3. examine for agglutination If + presence of cold Abs
phase 2 in tube test
37C incubation, detect warm reactive Ab
describe the steps of Phase 2 in tube test
enhancing substances can be added pt serum + reagent RBC + enhancing subs mixed incubate at 37 degrees and centrifuge
when checking cells for AGT you have to do what
follow each negative AHG tube test with a control system of IgG coated red cells
what is AGT aka
coombs test
what is a DAT
direct antiglobulin test
what does DAT detect
detects patients RBC that have already sensitized with IgG or complement in vivo
what does iAT detect
Detects anti RBC Abs in patient serum In vitro coating of RBCs with antibody or complement
what are tubeless tests
Gel column agglutiation solid phase RBC adherance tests
Gel testing positive result
agglutinated cells are too large to enter the gel matrix and remain at top
is this positive or negative
Positive for agglutination
is this positive or negative
negative for agglutination
positive solid phase test
indicator RBCs diffusely adhere over the well
