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IBI Test 3 > B3.050 - Prework Pre-Transfusion Workup > Flashcards

B3.050 - Prework Pre-Transfusion Workup Flashcards

1
Q

What is the endpoint of al serological tests

A

agglutination

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2
Q

what are the 2 steps of serological tests

A

sensitization agglutination

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3
Q

what is sensitization

A

Abs binding to RBCs surface Ag

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4
Q

what is agglutination

A

sensitized RBCs are bridged together to form the lattice

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5
Q

what is the AGT

A

antiglobulin test used to enhance agglutination usually require for clinical significant Ab detention

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6
Q

Type of RBC antibodies

A

IgM, IgG

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7
Q

Which is bigger IgM or IgG

A

IgM

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8
Q

what are cold antibodies

A

IgM - react best at 22 in IS phase

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9
Q

what do IgM antibodies do

A

Fix complement and cause intravascular hemolysis

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10
Q

what do IgG do and are they warm or cold

A

warm - react at 37 in AHG phase

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11
Q

what is the IS phase in a tube test

A

immediate spin phase When you put pt serum in a tube with a commercial reagent RBCs

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12
Q

describe the steps of phase 1 tube test

A
  1. mix 2. centrifuge @ RT 3. examine for agglutination If + presence of cold Abs
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13
Q

phase 2 in tube test

A

37C incubation, detect warm reactive Ab

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14
Q

describe the steps of Phase 2 in tube test

A

enhancing substances can be added pt serum + reagent RBC + enhancing subs mixed incubate at 37 degrees and centrifuge

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15
Q

when checking cells for AGT you have to do what

A

follow each negative AHG tube test with a control system of IgG coated red cells

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16
Q

what is AGT aka

A

coombs test

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17
Q

what is a DAT

A

direct antiglobulin test

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18
Q

what does DAT detect

A

detects patients RBC that have already sensitized with IgG or complement in vivo

19
Q

what does iAT detect

A

Detects anti RBC Abs in patient serum In vitro coating of RBCs with antibody or complement

20
Q

what are tubeless tests

A

Gel column agglutiation solid phase RBC adherance tests

21
Q

Gel testing positive result

A

agglutinated cells are too large to enter the gel matrix and remain at top

22
Q

is this positive or negative

A

Positive for agglutination

23
Q

is this positive or negative

A

negative for agglutination

24
Q

positive solid phase test

A

indicator RBCs diffusely adhere over the well

25
Q

oligosaccharide surface glycoproteins

A

ABO, I, P

26
Q

clinically significant allo-Ab cause what

A

hemolytic transfusion reaction and/or heomlytic disease of the fetus and neonate

27
Q

Most warm reactive allo antibodies are Ig___

A

IgG

28
Q

Most cold reactive allo-Ab are Ig__

A

IgM

29
Q

ABO is what type of Ig

A

IgM

30
Q

people with type O blood have what Ab in plasma

A

anti A&B, and anti AB

31
Q

The chance we cn aget Ag-negative RBC is

A

1-indicence of positivity

32
Q

which type of Ags are more clinically significant

A

protein, bc they are active at body temp

33
Q

what does blood type mean

A

which A, B and D antigens are present on a patients RBC

34
Q

whats a forward test

A

testing RBC with commercial anti-A, anti-B, anti-D

35
Q

Reverse test

A

patients plasma are miced with commercial testing cells (A+RBC and B+RBC)

36
Q

Ab screening is for

A

checking whether there are unexpected non-ABO antibody in the recipietn plasmabesides Anti-A or Anti-B

37
Q

if the Abs react in RT IS then

A

you dont need to worry about it becuase they wont react in body temp

38
Q

if antibodies react at 37 degrees then

A

they are warm antibodies and would react at room temp

39
Q

crossmatching

A

confirming theres no reaction between pt RBC and transfusion plasma

40
Q
A
41
Q

Crossmatching is mandatory when

A

the need of tranfusion is NOT urgent

42
Q

if the Ab screen is negative then you need to

A

confirm ABO compatibility and you dont need to worry about antibodies

43
Q

if the patient cross match has antibodies you have to

A

do complete XM

IBI Test 3 (16 decks)

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