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MTAP 1 FINALS > AUBF Chemical Urine Examination > Flashcards

AUBF Chemical Urine Examination Flashcards

1
Q

Benedict’s test does not detect

A

sucrose

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2
Q

Principle of benedict’s test

A

copper reduction

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3
Q

Reporting of Benedict’s test: No change in color (Blue solution)

A

Negative

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4
Q

Reporting of Benedict’s test: Green opacity, no precipitate

A

trace (+/-)

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5
Q

Reporting of Benedict’s test: Green solution with yellow precipitate

A

Positive (+)

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6
Q

Reporting of Benedict’s test: Green to yellow solution with yellow precipitate

A

Positive (++)

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7
Q

Reporting of Benedict’s test: Muddy orange solution with yellow precipitate

A

Positive (+++)

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8
Q

Reporting of Benedict’s test: Orange to brick red precipitate

A

Positive (++++)

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9
Q

Benedict’s test reaction result:

Cupric Ions (Blue Solution) + Reducing Sugar → (heat alkali) →

A

Carboxylic Acid + Cu2O (Cuprous Oxide) (Brick-Red precipitate)

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10
Q

SULFOSALICYLIC ACID PRECIPITATION (SSA) TEST Principle

A

Precipitation of urine protein by strong acid

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11
Q

Classic test for differentiation of urobilinogen and porphobilinogen

A

Watson-Schwartz Test

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12
Q

Butanol location in Watson-Schwartz Test

A

upper

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13
Q

Chloroform location in Watson-Schwartz Test

A

Lower

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14
Q

Turbidity grade and protein range:

No increase in turbidity

A

Negative: <6

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15
Q

Turbidity grade and protein range: Noticeable turbidity

A

Trace: 6-30

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16
Q

Turbidity grade and protein range: Distinct turbidity, no granulation

A

1+ : 30-100

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17
Q

Turbidity grade and protein range: Turbidity, granulation, no flocculation

A

2+: 100-200

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18
Q

Turbidity grade and protein range: turbidity, granulation, flocculation

A

3+: 200-400

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19
Q

Turbidity grade and protein range: clumps of protein

A

4+: >400

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20
Q

Analyte: Urobilinogen

Chloroform and Butanol result

A

Chloroform and Butanol: soluble

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21
Q

Analyte: Porphobilinogen

Chloroform and Butanol result:

A

C and B: Insoluble

22
Q

Analyte: other ehrlich reacting compounds

Chloroform and Butanol result:

A

C: Insoluble
B: Soluble

23
Q

Obsolete urine chemical test and analyte end result:

Heat and Acetic acid
Nitric Acid Test for albumin/protein

A

Turbidity

24
Q

Obsolete urine chemical test and analyte end result:

Benedict Test for Glucose (other reducing sugar)

A

Brick Red Precipitate

25
Q

Obsolete urine chemical test and analyte end result:

Heat Precipitation Test (Bence Jones Protein)

A

Turbidity

26
Q

Obsolete urine chemical test and analyte end result:

Rothera for ketones

A

Red purple color

27
Q

Obsolete urine chemical test and analyte end result:

Gunning for Ketones

A

Iodoform Crystals (microscopic)

28
Q

Obsolete urine chemical test and analyte end result:

Gerhadt for Ketones

A

Bordeaux Red Color

29
Q

Obsolete urine chemical test and analyte end result:

Seliwanoff for Levulose

A

Orange to Red Color

30
Q

Obsolete urine chemical test and analyte end result:

Rubner for Lactose

A

Brick Red Precipitate

31
Q

Obsolete urine chemical test and analyte end result:

Gmelin for Bile

A

Play of Colors (Yellow, Red, Green)

32
Q

Obsolete urine chemical test and analyte end result:

Smith for Bile

A

Green Ring

33
Q

Obsolete urine chemical test and analyte end result:

Erlich for Urobilinogen

A

Cherry Red

34
Q

Obsolete urine chemical test and analyte end result:

Schlesinger for Urobilin

A

Fluorescent Green

35
Q

Obsolete urine chemical test and analyte end result:

Obermayer for Indican

A

Indigo blue to Indigo red

36
Q

Obsolete urine chemical test and analyte end result:

Gualac for Hemoglobin

A

Blue color (junction)

37
Q

Obsolete urine chemical test and analyte end result:

Ammonium sulfate for Hemoglobin

A

Precipitation of HGB

38
Q

Obsolete urine chemical test and analyte end result:

Thormalen for melanin

A

Prussian blue color

39
Q

Obsolete urine chemical test and analyte end result:

Sulkowitch for calcium

A

Turbidity

40
Q

Obsolete urine chemical test and analyte end result:

Fantus for chloride

A

white precipitate

41
Q

Micral test normal protein range:

A

<10mg/dL or 100 mg/24 hours

42
Q

Microalbuminuria range for micral test:

A

20-200 mg/L

43
Q

Albuminuria range for micral test:

A

> 200 mg/L

44
Q

Indication of microalbuminuria and albuminuria

A

Kidney problems

45
Q

Principle of Microalbumin Testing (Immunologic Testing)

A

Enzyme Immunoassay

46
Q

Sensitivity of Micral test

A

0-10 mg/dL

47
Q

Reagents for Microalbumin testing

A

Gold-labeled antibody

B-Galactosidase

Chlorophenol red galactoside

48
Q

Interference for Micral Test

A

False-negative: Dilute urine

49
Q

Immunodip principle

A

Immunochromographics

50
Q

Sensitivity for Immunodip

A

1.2 to 8.0 mg/dL

51
Q

Reagents for immunodip

A

Antibody-coated blue latex particles

MTAP 1 FINALS (16 decks)

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