Antibody Flashcards
An antibody Fab contains
a. complementarity determining regions.
b. H and L chain variable regions.
c. one antigen binding region.
d. one H-L interchain disulfide bond.
e. all of the above.
e. all of the above.
Myeloma proteins are
a. abnormally formed antibodies secreted from cancerous plasma cells.
b. cancerous plasma cells that divide without requiring antigen activation.
c. cell lines that secrete specific antibodies for a short time, then die.
d. homogeneous antibody molecules secreted by plasma cell tumors.
e. protein signaling molecules that make a plasma cell become a multiple myeloma.
d. homogeneous antibody molecules secreted by plasma cell tumors.
The regions of the antibody molecule which contribute MOST to the affinity of the antibody for antigen are the
a. CDR.
b. Fab regions.
c. Fc regions.
d. framework regions.
e. hinge regions.
a. CDR.
Antibody Fc fragments contain
a. antigen-binding sites.
b. CDR.
c. complement-binding sites.
d. framework residues.
e. light chain variable domains.
c. complement-binding sites.
The immunoglobulin isotype is determined by the
a. antigen specificity.
b. H chain constant region.
c. L chain variable region.
d. number of antigen-binding sites.
e. number of VH domains.
b. H chain constant region.
Which statement about antigen epitopes is FALSE?
a. An epitope may be shared by two different antigens.
b. A protein molecule usually contains multiple epitopes.
c. B cells bind only processed antigen epitopes.
d. Epitopes may be linear (composed of sequential amino acids) or assembled by protein folding from amino acids far apart in the protein primary amino acid sequence.
e. Some epitopes are more immunogenic than others.
c. B cells bind only processed antigen epitopes.
An example of an antigen epitope from an infectious organism would be
a. a bacterial endotoxin (LPS) molecule.
b. a fungal cell wall protein.
c. a peptide on the surface of a virus capsid protein.
d. a whole virus.
e. All of the above are antigen epitopes.
c. a peptide on the surface of a virus capsid protein.
Antibody affinity for antigen depends on
a. the antibody isotype.
b. the complementary shape and charge of each antibody V region for its antigen epitope.
c. the number of Fab regions in each antibody molecule.
d. whether the antibody is in the serum or on the cell surface.
e. whether the light chains are kappa or lambda.
b. the complementary shape and charge of each antibody V region for its antigen epitope.
Avidity
a. is a pathogenic agent, causing a very serious disease.
b. occurs when the ratio of antibody to antigen is optimal.
c. refers to the strength of interactions between a multivalent antibody and a multivalent antigen.
d. results in a loss of antibody reactivity.
e. results in cross-reactivity when antibody binds two different antigens.
c. refers to the strength of interactions between a multivalent antibody and a multivalent antigen.
A colleague sends you an antibody to polio virus capsid protein. You perform equilibrium dialysis on the antibody to measure its affinity. Plotting r/c versus r gives you a curved line with K= 2.5 X 108 L/mole and an r intercept of 4. From these results, you conclude that the antibody is probably
a. a cross-reactive antibody.
b. a monoclonal anti-polio virus antibody.
c. a polyclonal IgG antibody.
d. IgA anti-polio virus.
e. not specific for polio virus.
d. IgA anti-polio virus.
Allotypic determinants are
a. constant region determinants that distinguish each Ig class and subclass within a species.
b. expressed only from the paternal chromosome.
c. generated by the conformation of antigen-specific VH and VL sequences.
d. Not immunogenic in individuals who do not have that allotype.
e. amino acid differences encoded by different alleles for the same H or L chain locus.
e. amino acid differences encoded by different alleles for the same H or L chain locus.
Which of the following is NOT a characteristic of IgG?
a. It contains 2 g and 2 L chains
b. It crosses the placenta.
c. It is the predominant immunoglobulin in blood, lymph, and peritoneal fluid.
d. It is the largest of all the Igs.
e. Its L chains are either k or l.
d. It is the largest of all the Igs.
Human serum IgA is isolated and injected into a rabbit. The rabbit anti-IgA antibodies will react against all of the following EXCEPT human
a. a chain.
b. IgG.
c. k chain.
d. l chain.
e. secretory component.
e. secretory component.
You have purified some Fab from an IgG myeloma protein. Under appropriate conditions, you could use this Fab to generate antibodies to
a. both k and l chain.
b. g chain hinge region.
c. J chain.
d. g chain allotypic determinants.
e. the idiotype of this myeloma.
e. the idiotype of this myeloma.
The Ig isotype which would be most important for neutralizing polio virus before it could infect intestinal cells would be
a. secretory IgA.
b. serum IgA.
c. serum IgD.
d. serum IgG.
e. membrane IgM.
a. secretory IgA.
