Amniotic Fluid

Question 1

HDN specimens must be protected from the

Answer 1

Light

Question 2

Specimens for fetal lung maturity (FLM) are delivered how?

Answer 2

On ice and refrigerated or frozen

Question 3

Amniotic fluid specimens for cytogenic testing are kept at

Answer 3

RT and delivered immediately

Question 4

Colorless amniotic fluid

Answer 4

Normal

Question 5

Blood-streaked amniotic fluid

Answer 5

Trauma, traumatic tap

Question 6

Yellow amniotic fluid

Answer 6

Bilirubin, HDN

Question 7

Dark green amniotic fluid

Answer 7

Meconium

Question 8

Dark red-brown amniotic fluid

Answer 8

Fetal death

Question 9

HDN: spectrophotometric analysis at optical densities what

Answer 9

Between 365 and 550 nm

Question 10

Neural tube defects detected first by

Answer 10

Maternal serum a-fetoprotein

Question 11

Neural tube defects: amniotic fluid AFP are measured first between ____________ and compared to maternal serum levels

Answer 11

Gestational weeks 12-15

Question 12

Report both serum and fluid AFP levels in

Answer 12

Multiple of the mean (MOM)

Question 13

Abnormal AFP value

Answer 13

Two time the laboratory median value in both serum and fluid

Question 14

Positive tests fetal distress / neural tube defects are followed by measuring

Answer 14

Amniotic acetylcholinesterase

Question 15

Decreased lung surfactant causes

Answer 15

Respiratory distress syndrome

Question 16

Primary lung surfactants are

Answer 16

Lecithin, sphingomyelin,

and phosphatidylglycerol.

Question 17

Immunologic agglutination test for phosphatidylglycerol

Answer 17

Aminostat-FLM

*not affected by meconium and blood contamination.

Question 18

The storage form of phospholipids in the lungs

Answer 18

Lamellar bodies

Question 19

Lamellar bodies are secreted by

Answer 19

Type II pneumocytes

Question 20

Lamellar bodies enter the amniotic fluid at _____________

Answer 20

26 weeks of gestation

Question 21

Lamellar bodies normal value

Answer 21

32000 to 35400.

*fluid can be run through the platelet counting chamber of automated cell counters

Question 22

An optical density measurement of amniotic fluid at ________ of ________ is also normal

Answer 22

650 nm; 0.150

Question 23

Amniotic fluid collected by

Answer 23

Amniocentesis