Amniotic Fluid Flashcards
HDN specimens must be protected from the
Light
Specimens for fetal lung maturity (FLM) are delivered how?
On ice and refrigerated or frozen
Amniotic fluid specimens for cytogenic testing are kept at
RT and delivered immediately
Colorless amniotic fluid
Normal
Blood-streaked amniotic fluid
Trauma, traumatic tap
Yellow amniotic fluid
Bilirubin, HDN
Dark green amniotic fluid
Meconium
Dark red-brown amniotic fluid
Fetal death
HDN: spectrophotometric analysis at optical densities what
Between 365 and 550 nm
Neural tube defects detected first by
Maternal serum a-fetoprotein
Neural tube defects: amniotic fluid AFP are measured first between ____________ and compared to maternal serum levels
Gestational weeks 12-15
Report both serum and fluid AFP levels in
Multiple of the mean (MOM)
Abnormal AFP value
Two time the laboratory median value in both serum and fluid
Positive tests fetal distress / neural tube defects are followed by measuring
Amniotic acetylcholinesterase
Decreased lung surfactant causes
Respiratory distress syndrome
Primary lung surfactants are
Lecithin, sphingomyelin,
and phosphatidylglycerol.
Immunologic agglutination test for phosphatidylglycerol
Aminostat-FLM
*not affected by meconium and blood contamination.
The storage form of phospholipids in the lungs
Lamellar bodies
Lamellar bodies are secreted by
Type II pneumocytes
Lamellar bodies enter the amniotic fluid at _____________
26 weeks of gestation
Lamellar bodies normal value
32000 to 35400.
*fluid can be run through the platelet counting chamber of automated cell counters
An optical density measurement of amniotic fluid at ________ of ________ is also normal
650 nm; 0.150
Amniotic fluid collected by
Amniocentesis
