9 Flashcards
RNA primer?
DNA P can only add a new base to an excisting chain by attaching to a free 3’ OH group in RNA primer.
DNA polymerase III?
The primary DNA polymerase that performs the majority of the DNA replication.
DNA Polymerase I?
To remove the RNA primers used during DNA replication by slowly breaking the primer and transcribing that gap.
dNTPs?
The four triphosphate monomers for each nucleotide base.
Origin of replication (OriC)?
Where replication starts.
How many origin of replications are there in Eukaryotes?
1<
During DNA replication, each strand is a?
Leading and lagging.
Problems of DNA replication? (3•)
•How to seperate the DNA as needed
•How to make primers
•Allow synthesis to happen simultaneously in both directions on both strands.
The separation of strands during DNA replication uses? (2•)
•Helicase
•Topoisomerase
Topoisomerase?
Cuts a strand and unwinds the end and puts it together to release the tension produced by helicase.
How to make DNA primers?
It uses RNA primase (a type of RNA polymerase) because it does not need a primer for itself.
How many bases are between RNA primase and the opening of the replication fork in the lagging strand?
About 200 bases.
DNA ligase?
The opening between the fragments are sealed up by DNA ligase since DNA polymerase I cannot make phosphodiester bonds.
DNA polymerase I?
To replicate the opening where RNA primase resided.
Single stranded binding protein?
To prevent separated DNA strands inside the replication fork to get together.
