8.2 DNA Replication Flashcards
Helicase
Separates the DNA strands
Primase
Lays down the RNA primer
Ligase
Glues together the Okazaki Fragments
DNA Polymerase III
The main DNA builder, makes 1,000 bases every second
DNA Polymerase I
Removes RNA primers, corrects mistakes, reduces errors from 1/10,000 to 1/100,000,000, makes 20 beses every second
Which direction does the leading strand go?
Toward the replication fork
Which direction does the lagging strand go?
Away from the replication fork
Which direction does DNA replication happen?
From the 5’ end to the 3’ end
What is the parent strand?
The original DNA strand that is being replicated
What are Okazaki Fragments?
Pieces of the lagging strand
What is the first step of replication?
Nucleotides start as a nucleoside, which has 3 phosphate groups
What is the second step of replication?
The nucleoside is turned into a nucleotide and 2 phosphate groups
What is the third step of replication?
DNA Polymerase III attaches 2 phosphates to a new nucleotides, making a nucleoside
What is the fourth step of replication?
Repeat the first three steps until the DNA is replicated.