5.1 - intro to bacteria Flashcards

1
Q

shapes of bacteria

A

cocucs, rods, comma shaped, spiral shaped

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2
Q

why is it better for bacteria to have a small size

A
  • rapid metab for quick diffusion

- leading to rapid turnover of sugars, AA and nucleotides

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3
Q

types of micorbiological media

A

liquid culture, solid media, selective media and differential media, defined medium and complex

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4
Q

what is selective media

A
  • Used for the growth of only selected microorganisms
  • so for antibiotic resistance, you inhibit the growth of the bacteria that doesnt have resistance but allows the bacteria with resistance to grow
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5
Q

what is differential media

A

can distinguish between diff bacteria

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6
Q

what nutrirents does mirciobioligcal media need

A

Carbon, nitorgen, oxygen, phosphorus and mineral

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7
Q

what pH does bacteria exhibit

A

7.4

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8
Q

types of bacteria regarding o2

A

aerobic, anaerobic

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9
Q

types of anareobic bacteria and their function

A

aerotolerant - can toleratw O2 and grow in air

obligate - O2 inhibits the growth and kills the bacteria

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10
Q

how can obligate anaerobes survive if they get killed in the air ?

A

forms spores so its like a dormant form of them

- can grow oncethe conditions are favourable

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11
Q

phases in bacterial growth curve

A
  1. lag
  2. expotential
  3. stationary
  4. death
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12
Q

what happens during lag phase

A
  • bacteria adapts to the new environment so their metabolism starts to happen
  • slow phase
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13
Q

what happens in expotential phase

A
  • rapid cell growth and metab

- nutirents run out and waste products accum

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14
Q

what happens during stationary phase

A
  • no growth so prepare to survive so the nutrients are used up
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15
Q

groups of bacteria regarding the staining

A

gram neg and gram pos

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16
Q

color of gram - and + bacteria

A

+ > purple

-> pink to red

17
Q

difference in colour for gram bacteria

A

gram pos - has no outer membrane

gram neg - has outer membrane which acts as a barrier towards antiobiotics

18
Q

experiments for bacterial infection in clinical setting

A
  • staining
  • agar sample through mass spectrometer
  • antigen detection assay
  • PCR DNA - visual on agarose gel
  • DN sequenceing