4.8 Reagent Strip testing (Bilirubin) Flashcards
Principle for bilirubin Reagent strip:
a. Sorensen’s Protein Error of Indicators
b. Double sequential enzyme reaction
c. Double Indicator Reaction
d. Na(Sodium) Nitroprusside reaction
e. Pseudoperoxidase activity of heme
f. Diazo reaction
g. Greiss’ Reaction
h. Ehrlich’s reaction
f
All sample containing heme be it hemoglobin or myoglobin, will produce a positive result for bilirubin
T or F
T
Reagents for Bilirubin in Multistix:
a. M&C: Methyl Red & Bromthymol Blue
b. Tetrabromophenol blue
c. GOD
d. Sodium nitroprusside
e. Dichloroaniline diazonium salt
f. Dichlorobenzene diazonium salt
g. p-arsanilic acid tetrahydrobenzo-quinolinol
h. Derivative pyrrole amino acid ester
e
Reagents for Bilirubin in Chemstrip:
a. M&C: Methyl Red & Bromthymol Blue
b. Tetrabromophenol blue
c. GOD
d. Sodium nitroprusside
e. Dichloroaniline diazonium salt
f. Dichlorobenzene diazonium salt
g. p-arsanilic acid tetrahydrobenzo-quinolinol
h. Derivative pyrrole amino acid ester
f
Color reaction for Bilirubin:
a. Tan, or pink violet
b. Brown, or pink violet
c. Tan, or brown
a
Reading time for Bilirubin ?
a. 10s
b. 30s
c. 60s
b
Sensitivity/Detection Limit:
0.4-0.8 mg/dL
a. Multistix
b. Chemstrip
a
Sensitivity/Detection Limit:
0.5 mg/dL
a. Multistix
b. Chemstrip
b
Sources of Interference for bilirubin:
Highly pigmented urine
a. F+
b. F-
a
Sources of Interference for bilirubin:
Expose to light
a. F+
b. F-
b
Sources of Interference for bilirubin:
Phenazopyridine
a. F+
b. F-
a
Sources of Interference for bilirubin:
Indican
a. F+
b. F-
a
Sources of Interference for bilirubin:
Metabolites of iodine
a. F+
b. F-
a
Sources of Interference for bilirubin:
High concentration of nitrite
a. F+
b. F-
b
Sources of Interference for bilirubin:
Ascorbic acid
a. F+
b. F-
b
