3.3 Chemical Examination of Urine (Protein) Flashcards
Normal Values for protein?
<30mg/dl or <150 mg/day
The glomerular filtration barrier is impermeable to proteins in general.
T or F
T
Overflow proteinuria
a. Pre-renal
b. Renal (Glomerular)
c. Renal (Tubular)
d. Post renal
a
Low molecular weight protein in the blood that overflows in the urine which is excreted readily
a. Pre-renal
b. Renal (Glomerular)
c. Renal (Tubular)
d. Post renal
a
May relate to Fanconi Syndorme (Failure of PCT reabsorption)
a. Pre-renal
b. Renal (Glomerular)
c. Renal (Tubular)
d. Post renal
c
Diabetic nephropathy
a. Pre-renal
b. Renal (Glomerular)
c. Renal (Tubular)
d. Post renal
b
What is the earliest indicator for Diabetic Nephropathy?
Microalbuminuria
Intravascular Hemolysis / Hemolytic disorder
a. Pre-renal
b. Renal (Glomerular)
c. Renal (Tubular)
d. Post renal
a
Hemoglobin is the protein of interest
a. Pre-renal
b. Renal (Glomerular)
c. Renal (Tubular)
d. Post renal
a
Muscle injury (Myoglobin)
a. Pre-renal
b. Renal (Glomerular)
c. Renal (Tubular)
d. Post renal
a
Inflammation (APR)
a. Pre-renal
b. Renal (Glomerular)
c. Renal (Tubular)
d. Post renal
a
Multiple myeloma (Light chain of IgG)
a. Pre-renal
b. Renal (Glomerular)
c. Renal (Tubular)
d. Post renal
a
Bence Jones Protein from Multiple myeloma
a. Pre-renal
b. Renal (Glomerular)
c. Renal (Tubular)
d. Post renal
a
Bence Jones is characterized with unique thermal sensitivity which has a ______ C and disappears at _____ C
40-60
100
Lower UTI
a. Pre-renal
b. Renal (Glomerular)
c. Renal (Tubular)
d. Post renal
d
In SSA TEST / Cold protein precipitation, it is a traditional test for Protein detrmination which differs from protein reagent strip test in that it detects BOTH ________ and _______
Albumins and globulins
SSA test does not require heating
T or F
T
Procedure for SSA test:
__mL of __% SS is added to __ mL of ____________ urine
3
3
3
Centrifuge
One of the important observation after adding SSA is turbidity.’
T or F
T
Degree of turbidity:
Noticeable tubidity (6-30)
a. Neg
b. Trace
c. 1+
d. 2+
e. 3+
f. 4+
b
Degree of turbidity:
Turbidity w/ Granulation (100-200)
a. Neg
b. Trace
c. 1+
d. 2+
e. 3+
f. 4+
d
Degree of turbidity:
Turbidity with granulation and flocculation (200-400)
a. Neg
b. Trace
c. 1+
d. 2+
e. 3+
f. 4+
e
Degree of turbidity:
Distinct Turbidity with no granulation (30-100)
a. Neg
b. Trace
c. 1+
d. 2+
e. 3+
f. 4+
c
Degree of turbidity:
Clumps of protein(>400)
a. Neg
b. Trace
c. 1+
d. 2+
e. 3+
f. 4+
f
Degree of turbidity:
<6mg/dL
a. Neg
b. Trace
c. 1+
d. 2+
e. 3+
f. 4+
a
Normal value of protein is from Neg only
T or F
F
Neg to trace
Micral test is based on Enzyme immunoassay
Immunodip is based on immunochromatographic technique
T or F
T
Test for microalbuminuria includes:
Micral test
Immunodiip
Albumin/Creatinine Ratio
Albumin Excretion Rate
T or F
T
Significant result:
ACR: 30-300 mg
AER: 20-200 / mins or 30-300mg/24 hrs
T or F
T