4.10 Reagent Strip testing (Nitrite) Flashcards
Principle for Nitrite Reagent strip?
a. Sorensen’s Protein Error of Indicators
b. Double sequential enzyme reaction
c. Double Indicator Reaction
d. Na(Sodium) Nitroprusside reaction
e. Pseudoperoxidase activity of heme
f. Diazo reaction
g. Greiss’ Reaction
h. Ehrlich’s reaction
g
Reagents for Nitrite reagent strips in multistix:
a. M&C: Methyl Red & Bromthymol Blue
b. Tetrabromophenol blue
c. GOD
d. Sodium nitroprusside
e. Dichloroaniline diazonium salt
f. Pdimethylaminobenzaldehyde
g. p-arsanilic acid tetrahydrobenzo-quinolinol
h. Sulfanilamide-hydroxy THBQ
g
Reagents for Nitrite reagent strips in Chemstrip:
a. M&C: Methyl Red & Bromthymol Blue
b. Tetrabromophenol blue
c. GOD
d. Sodium nitroprusside
e. Dichloroaniline diazonium salt
f. Pdimethylaminobenzaldehyde
g. p-arsanilic acid tetrahydrobenzo-quinolinol
h. Sulfanilamide-hydroxy THBQ
h
Reagents for Nitrite reagent strips:
→ Produces diazonium salt that then reacts with nitrite
a. p-arsanilic acid tetrahydrobenzo-quinolinol
b. Sulfanilamide-hydroxy THBQ
a
Color reaction for Nitrite reagent strip:
a. Pink
b. Yellow
c. Brown
a
Reading time for Nitrite:
a. 10s
b. 30s
c. 60s
c
Sensitivity/Detection Limit:
0.06-0.1 mg/dL
a. Multistix
b. Chemstrip
a
Sensitivity/Detection Limit:
0.5 mg/dL
a. Multistix
b. Chemstrip
b
Sources of Interference for nitrite:
Old specimen (nitrate is reduced to nitrite by bacteria)
a. F+
b. F-
a
Sources of Interference for nitrite:
Non-NO3-reducing bacteria
a. F+
b. F-
b
Sources of Interference for nitrite:
High SG
a. F+
b. F-
b
Sources of Interference for nitrite:
Bacterial overgrowth
a. F+
b. F-
b
nagiging nitrogen gas
Sources of Interference for nitrite:
Ascorbic acid
a. F+
b. F-
b
Required contact time for nitrite is at least ___ hrs (Amount of time that urine should be held in the bladder)
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