4.11 Reagent Strip testing (Leukocyte Esterase)

Question 1

Principle for LE reagent strips:

a. Sorensen’s Protein Error of Indicators
b. Double sequential enzyme reaction
c. Double Indicator Reaction
d. Na(Sodium) Nitroprusside reaction
e. Granulocytic Esterase Reaction coupled with Diazo Reaction
f. Diazo reaction
g. Greiss’ Reaction
h. Ehrlich’s reaction

Answer 1

e

Question 2

Reagents for LE strip test in multistix:

a. M&C: Methyl Red & Bromthymol Blue
b. Tetrabromophenol blue
c. GOD
d. Sodium nitroprusside
e. Dichloroaniline diazonium salt
f. Indoxycarbonic acid ester
g. p-arsanilic acid tetrahydrobenzo-quinolinol
h. Derivative pyrrole amino acid ester

Answer 2

h

Question 3

Reagents for LE strip test in Chemstrip:

a. M&C: Methyl Red & Bromthymol Blue
b. Tetrabromophenol blue
c. GOD
d. Sodium nitroprusside
e. Dichloroaniline diazonium salt
f. Indoxycarbonic acid ester
g. p-arsanilic acid tetrahydrobenzo-quinolinol
h. Derivative pyrrole amino acid ester

Answer 3

f

Question 4

Indoxycarbonic acid ester, Ester is a ___________ since esterase is being detected

Answer 4

Substrate

Question 5

Diazonium salt is present on both reagent in M and C

T or F

Answer 5

T

Question 6

Color reaction for LE?

a. Purple
b. Red
c. Pink

Answer 6

a

Question 7

Reading time for LE?

Answer 7

120s

Question 8

Sensitivity/Detection Limit:

5-10 WBCs/Hpf

a. Multistix
b. Chemstrip

Answer 8

a

Question 9

Sensitivity/Detection Limit:

10-25 WBCs/Hpf

a. Multistix
b. Chemstrip

Answer 9

b

Question 10

Sources of Interference in LE:

Strong OAs, formalin, highly pigmented urine, nitrofurantoin

a. F+
b. F-

Answer 10

a

Question 11

Sources of Interference in LE:

↑ protein, glucose, oxalic acid, ascorbic acid, gentamicin, cephalosporin, tetracycline

a. F+
b. F-

Answer 11

b