4.7 Reagent Strip testing (Blood) Flashcards
Principle of blood
a. Sorensen’s Protein Error of Indicators
b. Double sequential enzyme reaction
c. Double Indicator Reaction
d. Na(Sodium) Nitroprusside reaction
e. Pseudoperoxidase activity of heme
f. Diazo reaction
g. Greiss’ Reaction
h. Ehrlich’s reaction
e
Myoglobinuria also tests positive for the reagent strip test for blood because it contains myoglobin, which is heme containing
T or F
T
Reagents for multistix in blood?
a. M&C: Methyl Red & Bromthymol Blue
b. Tetrabromophenol blue
c. GOD
d. Sodium nitroprusside
e. Dichloroaniline diazonium salt
f. Pdimethylaminobenzaldehyde
g. p-arsanilic acid tetrahydrobenzo-quinolinol
h. Derivative pyrrole amino acid ester
i. Diisopropyl-benzenedihydro-peroxide
j. Dimethyl-dihydroperoxyhexane Tetramethylbenzidine
i
Reagents for Chemstrip in blood?
a. M&C: Methyl Red & Bromthymol Blue
b. Tetrabromophenol blue
c. GOD
d. Sodium nitroprusside
e. Dichloroaniline diazonium salt
f. Pdimethylaminobenzaldehyde
g. p-arsanilic acid tetrahydrobenzo-quinolinol
h. Derivative pyrrole amino acid ester
i. Diisopropyl-benzenedihydro-peroxide
j. Dimethyl-dihydroperoxyhexane Tetramethylbenzidine
j
Components for Chemstrip in blood
(Dimethyl-dihydroperoxyhexane Tetramethylbenzidine), includes:
Diisipropyl-benzene
a. Lysing agent
b. Oxidizing agent
c. Chromogen
a
Components for Chemstrip in blood
(Dimethyl-dihydroperoxyhexane Tetramethylbenzidine), includes:
Tetramethylbenzidine
a. Lysing agent
b. Oxidizing agent
c. Chromogen
c
Components for Chemstrip in blood
(Dimethyl-dihydroperoxyhexane Tetramethylbenzidine), includes:
dihydro-peroxide
a. Lysing agent
b. Oxidizing agent
c. Chromogen
b
Tetramethylbenzidine is the chromogen common to both multistix and chemstrip
T or F
T
Color reaction of Blood in reagent strip:
a. Red-Yellow
b. Yellow - Red
c. BLue-green
c
Catalyst for color reaction in blood?
Heme
Reaction may be homogenous or speckled in blood reagent strip:
Hematuria
a. Speckled
b. Homogenous
a
Reaction may be homogenous or speckled in blood reagent strip:
Hemoglobinuria or Myoglobinuri
a. Speckled
b. Homogenous
b
Reading time for Blood Reagent strip?
a. 10s
b. 30s
c. 60s
c
Sensitivity/Detection Limit for blood reagent strip
a. 5-20 RBCs/mL
b. 5-30 RBCs/mL
c. 5-35 RBCs/mL
a
Sources of Interference:
Strong Oxidizing Agents
a. F +
b. F -
a