4.4 Reagent Strip testing (Protein)

Question 1

Principle: Presence of certain indicators that changes color in the presence of protein even if the pH remains constant

a. Sorensen’s Protein Error of Indicators
b. Double sequential enzyme reaction
c. Double Indicator Reaction
d. Na(Sodium) Nitroprusside reaction
e. Pseudoperoxidase activity of heme
f. Diazo reaction
g. Greiss’ Reaction
h. Ehrlich’s reaction

Answer 1

a

Question 2

Multistix reagent for Protein?

a. M&C: Methyl Red & Bromthymol Blue
b. Tetrabromophenol blue
c. GOD
d. Sodium nitroprusside
e. Dichloroaniline diazonium salt
f. Pdimethylaminobenzaldehyde
g. p-arsanilic acid tetrahydrobenzo-quinolinol
h. Derivative pyrrole amino acid ester
i. Tetracholorophenoltetrabromosulfon-phtalein

Answer 2

b

Question 3

Chemstrip reagent for protein

Multistix reagent for Protein?

a. M&C: Methyl Red & Bromthymol Blue
b. Tetrabromophenol blue
c. GOD
d. Sodium nitroprusside
e. Dichloroaniline diazonium salt
f. Pdimethylaminobenzaldehyde
g. p-arsanilic acid tetrahydrobenzo-quinolinol
h. Derivative pyrrole amino acid ester
i. Tetracholorophenoltetrabromosulfon-phtalein

Answer 3

i

Question 4

Buffer with pH in protein: what is the pH?

Answer 4

3.0

Question 5

Color Reaction for Protein:

____–>_____–>_____

a. Red, Yellow
b. Yellow, Blue-green
c. Blue, Yellow

Answer 5

b

Question 6

Reading time for protein reagent strip?

a. 10s
b. 30s
c. 60s

Answer 6

c

Question 7

Sensitivity/Detection Limit for protein:

15-30 mg/dl

a. Multistix
b. Chemstrip

Answer 7

a

Question 8

Sensitivity/Detection Limit for protein:

6 mg/dL

a. Multistix
b. Chemstrip

Answer 8

b

Question 9

Source of Interferences: (Protein)

↑pH – overrides the buffer

a. F-Positive
b. F-Negative

Answer 9

a

Question 10

Source of Interferences: (Protein)

Proteins other than albumin (e.g., globulins)

a. F-Positive
b. F-Negative

Answer 10

b

Question 11

Source of Interferences: (Protein)

→ Highly pigmented specimens, chlorhexidine, phenazopyridine,
QACs

a. F-Positive
b. F-Negative

Answer 11

a

Question 12

Source of Interferences: (Protein)

Loss of buffer – when pad is exposed to the specimen for too long

a. F-Positive
b. F-Negative

Answer 12

a

Question 13

Source of Interferences: (Protein)

Microalbuminuria

a. F-Positive
b. F-Negative

Answer 13

b

Question 14

Source of Interferences: (Protein)

↑ salt conc. – lowers sensitivity of the pad

a. F-Positive
b. F-Negative

Answer 14

b