4 Genetics - Transcription and Translation Flashcards
what is a codon?
a sequence of 3 bases on mRNA which codes for an amino acid
what is a genome?
a complete set of chromosomes/ genes in a cell , this includes mitochondrial and ribosomal chromosomes
what is a proteome?
the full range of proteins produced by the genome
what is mRNA
messenger RNA, made in the nucleus and contains codons, transfers the genetic code from the nucleus to a ribosome in the cytoplasm, it leaves the nucleus via a nuclear pore
what is tRNA?
contains anticodons and attaches to amino acids where it forms weak H bonds.
What is the main function of transcription?
Making RNA, this happens in the nucleus
Step 1 of transcription
RNA polymerase binds to promoter region on the DNA, this breaks the hydrogen bonds between bases to reveal the template strand.
Step 2 of transcription
Free RNA nucleotides found in the nucleoplasm pair in a complimentary manner to the template strand, forming phosphodiester bonds
Step 3 of transcription
RNA polymerase catalyses the rejoining of DNA strands
Step 4 of transcription
Pre - mRNA contains non coding introns, these are removed through a process called splicing, this joins the exons together, the mRNA then leaves via the nuclear pore
What is the main function of translation?
making an amino acid chain from mRNA
What type of ribosomes are in eukaryotic cells?
80s
Step 1 of translation
mRNA is attracted to the ribosome.
Step 2 of translation
tRNA arrives at the ribosome attached to an amino acid
Step 3 of translation
tRNA anticodon pairs to the complimentary codons on the mRNA