4 Genetics - Transcription and Translation Flashcards

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1
Q

what is a codon?

A

a sequence of 3 bases on mRNA which codes for an amino acid

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2
Q

what is a genome?

A

a complete set of chromosomes/ genes in a cell , this includes mitochondrial and ribosomal chromosomes

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3
Q

what is a proteome?

A

the full range of proteins produced by the genome

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4
Q

what is mRNA

A

messenger RNA, made in the nucleus and contains codons, transfers the genetic code from the nucleus to a ribosome in the cytoplasm, it leaves the nucleus via a nuclear pore

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5
Q

what is tRNA?

A

contains anticodons and attaches to amino acids where it forms weak H bonds.

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6
Q

What is the main function of transcription?

A

Making RNA, this happens in the nucleus

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7
Q

Step 1 of transcription

A

RNA polymerase binds to promoter region on the DNA, this breaks the hydrogen bonds between bases to reveal the template strand.

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8
Q

Step 2 of transcription

A

Free RNA nucleotides found in the nucleoplasm pair in a complimentary manner to the template strand, forming phosphodiester bonds

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9
Q

Step 3 of transcription

A

RNA polymerase catalyses the rejoining of DNA strands

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10
Q

Step 4 of transcription

A

Pre - mRNA contains non coding introns, these are removed through a process called splicing, this joins the exons together, the mRNA then leaves via the nuclear pore

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11
Q

What is the main function of translation?

A

making an amino acid chain from mRNA

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12
Q

What type of ribosomes are in eukaryotic cells?

A

80s

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13
Q

Step 1 of translation

A

mRNA is attracted to the ribosome.

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14
Q

Step 2 of translation

A

tRNA arrives at the ribosome attached to an amino acid

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15
Q

Step 3 of translation

A

tRNA anticodon pairs to the complimentary codons on the mRNA

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16
Q

Step 4 of translation

A

the first code is always AUG, this codes for amino acid methionine

17
Q

Step 5 of translation

A

Codons are always transcribed in 3s, each tRNA brings a new amino acid to the mRNA

18
Q

Step 6 of translation

A

tRNA detaches from amino acid as peptide bonds form between adjacent amino acids

19
Q

where does RNA polymerase bind to?

A

the promoter region on DNA