1 Cells - Cell centrifugation/ fractionation

Question 1

What is step 1?

Answer 1

Homogenisation
- tissue sample placed in an ice cold isotonic buffer
- ice cold = stop enzyme action
- isotonic = to prevent cell from bursting due to differing water
potentials
- buffer = to prevent organelles and proteins from becoming
denatured
-

Question 2

What is step 2?