3.7

Question 1

The equation to find the total magnification of a specimen

Answer 1

magnification of objective lens* magnification of eyepiece lens

Question 2

The equation to find the actual size of a specimen

Answer 2

actual size= image size/magnification

Question 3

Why is staining used?

Answer 3

To make it easier to identify types of cell or parts of cells under the microscope

Question 4

Name four stains that may be used to identify part of cells

Answer 4

1) Hematoxylin
2) Methylene blue
3) Acetocarmine
4) Iodine

Question 5

What parts of the cells does the 4 stains stain?

Answer 5

1) Hematoxylin- stains the nuclei of plant and animal cells purple, blue or ibrown
2) Methylene blue- Stains the nuclei of animal cells blue
3) Acetocarmine- Stains the chromosomes in dividing nuclei in both plant and animal cells
4) Iodine- Stains starch-containing material in plant cells blue-black

Question 6

What are the two features of any microscope that determine how clear the image is?

Answer 6

Magnification and resolution

Question 7

What is magnification?

Answer 7

Is a measure of how much bigger the image you see is when compared to the real object

Question 8

What is resolution?

Answer 8

Is a measure of how far apart two objects must be before we see them separately