2D - Gene Expression Flashcards
precursor messenger RNA (pre-mRNA)
The immediate product of transcription of a DNA sequence. Requires modifications before it can undergo translation.
transcription factor
Proteins that bind to the promoter region and control the functioning of RNA polymerase.
template strand
The strand of DNA transcribed by RNA polymerase to produce a complimentary pre-mRNA strand.
coding strand
The strand of DNA NOT transcribed by RNA polymerase, contains an identical sequence to the mRNA strand produced (except thymine is replaced with uracil in mRNA).
ribosome
An organelle made of rRNA and protein that is the site of protein synthesis. Can be free in the cytosol or attached to the RER.
5’ methyl-G cap
A molecule added to the 5’ end of pre-mRNA during RNA processing.
3’ poly-A tail
A chain of adenine nucleotides added to the 3’ end of pre-mRNA during RNA processing.
splicing
process where introns are cut out of a pre-mRNA molecule, and exons are joined together.
spliceosome
The enzyme that removes introns from the pre-mRNA molecule and joins exons together during RNA processing.
alternative splicing
The process where different exons may be spliced, resulting in a single gene producing multiple different mRNA strands.
anticodon
The sequence of three nucleotides on a tRNA molecule that recognises a specific sequence of three nucleotides (codon) on an mRNA stand.
exocytosis
A type of bulk transport that moves large substances out of a cell.