28 - Asfarviridae, Hepadnaviridae

Question 1

Asfarviridae

Answer 1

• Etymology: African Swine Fever
• Genus: Asfivrus genus
• Morphology:
  
  • ​Linear dsDNA
  • Enveloped
  • Isohedrical capsid
• Resistance: Strong
  
  • But in raw pork meat the virus survives for months!
• Biological properties:
  
  • Baltimore I. ↔ multiplication in the cytoplasm (viral enzymes)
  • Multiplication in lymphatic cells – buffy coat culture
  • Haemadsorption
  • Stenoxen
  • Reservoir (african): wart-hog
  • Transmission: contact, arbo
• Antigenic properties:​
  
  • Hidden protective antigen – no neutralizing antibodies!
  • No vaccines available
  • Persisting infections
  • Immunocomplex deposition – clinical signs
• Diagnosis:​
  
  • Clinical signs = classical swine fever – swine experiment
  • Isolation – buffy coat
  • IF, ELISA
• Afsvirus genus:
  
  • ​African swine fever:
    
    • Swine: hing morbidity and mortality
    • Acute: fever, aemorrhage (skin, organs)
    • Chronic: paralysis, wasting

Question 2

Hepadnaviridae​

Answer 2

• Etymology: Hepar, DNA
• Genera:
  
  • ​Orthohepadna genus (annet flaschard)
  • Avihepadnavirus genus (annet flaschard)
• Morphology:
  
  • ​Circular ss/dsDNA
  • Icosahedral capsid
  • Enveloped
• Resistance: Weak
• Biological properties:​
  
  • Baltimore VI! – replication with reverse transcriptase
  • Stenoxen viruses
  • Transmission: blood, sexual route, close contact
  • Chronic, persisting infections
• Antigenic properties:
  
  • ​HBsAg - protective
  • HBcAg
  • HBeAg
• Diagnosis: ELISA direct and indirect

Question 3

Hepadnaviridae

Orthohepadnvirus genus

Answer 3

• Human hepatitis B
  
  • Serumhepatitis
  • Hepatocarcinoma
• Woodchuck hepatitis
• Ground squirrel hepatitis
• Bat hepatitis
• Monkey hepatitis

Question 4

Hepadnaviridae

Avihepadnavirus genus

Answer 4

• Duck hepatitis (B)
  
  • ​Far east
  • Hepatitis
• Heron, parrot, hepatitis B viruses