25 - Papillomaviridae, Polyomaviridae, Adenoviridae

Question 1

Polyomaviridae

Answer 1

• Etymology:
  
  • Poly: many
  • Oma: tumor
• Genus: Polymavirus
• Morphology:
  
  • ​Circular
  • dsDNA
  • Superhelical
  • No envelope
• Biological property:
  
  • ​Baltimore I: cellular transcriptase
  • Oncogenic: SV40 model virus
  • Latency: into genome → provirus
  • Especially in kidney tubular cells → viruria
  • Airborne
• Resistance: ​Strong
• Diagnosis:
  
  • ​IF, EM, HAI
  • NA- hybridization, PCR

Question 2

Polyomaviridae

Name the different viruses

Answer 2

• Other adaptions:
  
  • ​Simian polymavirus SV40 (monkey and rodent tumors)
  • Bovine polymavirus
  • Goose polymavirus - nepthritis
• Human:​
  
  • ​BK virus:
    
    • ​80-100% seropositivity
    • No clinical symptoms or mild respiratory disease
    • Nephropathy in kidney transplat recipients
  • JC virus
    
    • ​​​50-90% seropositivity
    • No symptoms
    • Progressive multifocal leucoencephalopathy
      
      • Rare fatal demyelization
      • In immunosuppressed patients (AIDS 4%)

Question 3

Papillomaviridae

Answer 3

• Etymology:
  
  • Papilla: wart
  • Oma: tumor
• Genus: Papillomavirus
• Morphology:
  
  • ​Circular
  • dsDNA
  • No envelope
• Resistance: Strong
• Biological properties:
  
  • ​Baltimore I. - cellular transcriptase
  • Virus assembly in keratinized cells
    
    • No propagation in tissue cultures
  • Stenoxen (restricted host range, predilection sites)
  • Oncogenic – benignant tumors
  • Latency: episomal DNA
  • Contact, venereal transmission – cell-associated
• Antigenic properties:
  
  • Good antigens ↔ hidden for the immune system → autovaccines
  • No cross reactions
• Diagnosis:
  
  • ​Clinical sings, IF, EM
  • NA hybridization

Question 4

Papillomviridae

List the different genera

Answer 4

Papillomvirus genus

Question 5

Papillomaviridae

Papillomavirus genus

Answer 5

• ​​Warts, papillomas: benignant tumors - pre-malignant status
• Equine papillomatosis: head, nose
  
  • Transmitssion: bit, twitch
• Bovine paillomatosis 1-13: neck, back, body
  
  • Horse sarcoid
• Canine oral papillomatosis: oral mucosa, epulis
• • many other species
• Humans:
  
  • ​Skin papillomas - verruca vulgaris
  • Genital papillomas - ​sexually transmitted diesase
    
    • Juvenile laryngeal carcinomas
    • Cervical intraepithelial neoplasia
    • Vulval-, penile carcinomas
  • Cervical cancer
    
    • Caused by HPV 16, 18

Question 6

Adenovirdae

Answer 6

• Etymology: Adenos: adenoid glands
• Morphology:
  
  • ​Linear dsDNA
  • No envelope
  • Oncogenic
• Biological properties:
  
  • ​Baltimore I: cellular transcriptase
  • Stenoxen
  • CPE: nuclear inclusion bodies, rounding, lysis
  • Oncogenity: apoptosis inhibition
• Antigenic properties:
  
  • ​Good antigens
  • Cross reactions: AGID, CF, IF
  • Serotyping: VN, HAI
• Diagnosis:
  
  • ​Isolation
  • AGID, VN

Question 7

Adenovirdae

List the different genera

Answer 7

• Mastadenovirus genus (​Masto: breast)
• Aviadenovirus genus (Avis: bird)
• Atadenovirus genus (High A and T content of the genome)
• ​Siadenovirus genus (Sialidase enzyme gene)
• ​Ichtadenovirus genus (Fish adenoviruses)

Question 8

Adenovirus

Mastovirus genus

Answer 8

• Etymology: mastro: breast
• Pneumoenteritis
  
  • Calves and lambs
  • Bladder stone
• Infectious canine hepatitis (fox encephalitis)
• Canine laryngotracheitis
  
  • Kennel cough
• Human adenoviruses
  
  • Military disease

Question 9

Adenovirus

Aviadenovirus genus

Answer 9

• Etymology: avis: birds
• Chicke, turkey, goose, pigeon adenoviruses
• Inclusion body hepaitits
• Nephritis

Question 10

Adenovirus

Atadenovirus genus

Answer 10

High A and T content of the genome

• Pneumoenteritis of calves and lambs
• Egg drop syndrome (EDS)
• Snake adenoviruses

Question 11

Adenovirus

Atadenovirus

Answer 11

Sialidase enzyme gene

• Hemorrhagic enteritis
  
  • Turkey

Question 12

Adenovirus

​Ichtadenovirus genus

Answer 12

• Fish adenoviruses
• Sturgeon adenovirus