2.4 Catalase Enzyme Experiment Flashcards
1
Q
What is the IV in this experiment?
A
Temperature
2
Q
What is the DV in this experiment?
A
Volume of O2
3
Q
What are other variables that need to be controlled?
A
PH
Substrate concentration
Enzyme concentration
4
Q
- Set up boiling tubes with same volume and concentration of what?
A
Hydrogen peroxide
5
Q
- How do you keep PH constant, what does this do?
A
Adding buffer solution
Resists changes in PH when small amounts of acids and alkalis are added
6
Q
- What apparatus is set up to measure volume of O2 production?
A
Delivery tube and upside down measuring cylinder
7
Q
- Each boiling tube containing hydrogen peroxide is put where?
A
Water bath
8
Q
- What is different about each water bath?
A
Each set to different temperature
9
Q
- What else is put in a water bath other than hydrogen peroxide solution?
A
Tube containing catalase
10
Q
- How long do you wait for each enzyme to reach set temperature before removing from water bath?
A
5 minutes
11
Q
- Then use a pipette to add what?
A
The same volume and concentration of catalase to each tube
12
Q
- The record O2 production in the …
A
First minute reaction
13
Q
- How many times do you repeat the experiment?
A
3 times at each temperature
14
Q
- What do you then use the results to calculate?
A
Mean O2 production at each temperature
15
Q
- You can then calculate mean rate of reaction at each temperature, what is the calculation for this?
A
Volume of O2 produced ÷ time taken (cm3s-1)
