21: corona, arteri Flashcards
Nidovirales:
- familiy
- Coronaviridae
- Tobaniviridae
- Arteriviridae
- (Roniviridae, Mesoviridae)
Coronaviridae:
- subfamilies, genera
(Nidovirales)
Orthocoronavirinae: Alpha-, Beta-, Gamma-, Deltacoronavirus genus
Coronaviridae:
- Morphology
- Biological properties
- Antigenic properties
- Diagnosis
Morphology: - +ssRNA genome - ~80nm helical capsid - enveloped, ~100 nm Resistance: good pH resistance (pH 3-9), heat labile
Biological properties:
- Baltimore IV., subgenomic mRNA synthesis
- host specific – in vitro too
- CPE: not characteristic (rounding, lysis)
- HA: varies (HEV)
Antigenic properties:
- relatively good antigens
- cross-reactions within genera – „serogroups” (IF, ELISA)
Diagnosis:
- isolation (difficult) + IF
- clinical signs, IF, IEM
- indirect: ELISA, IF (cross-reactions), VN, (HAI)
Alphacoronavirus genus:
(Nidovirales, Coronaviridae, Orthocoronavirinae)
- Porcine transmissible gastroenteritis virus (TGE)
- -> piglets – severe enteritis, diarrhoea; respiratory form
- Porcine epidemic diarrhea virus (ED)
- Feline infectious peritonitis virus (FIP)
- -> immunocomplex disease
- -> wet form: ascites, peritonitis
- -> dry form: nephritis, uremia
- Canine coronavirus – enteritis, diarrhoea
- Human coronaviruses (229E, NL63)
- Bat coronaviruses
Betacoronavirus genus:
(Nidovirales, Coronaviridae, Orthocoronavirinae)
- Bovine coronavirus
- -> winter dysentery, calf diarrhoea
- Porcine hemagglutinating encephalomyelitis virus (HEV)
- Mouse hepatitis virus
- Canine respiratory coronavirus – kennel cough syndrome
- Human respiratory coronavirus
- Severe acute respiratory syndrome (SARS) virus
- -> human pneumonia
- -> surfactant production ↓ - atelectasia
- -> natural host: palm civet cat, bat
- Middle East respiratory syndrome (MERS) virus
- -> human pneumonia, camel reservoir
- Equine coronavirus
Gammacoronavirus genus:
(Nidovirales, Coronaviridae, Orthocoronavirinae)
- Avian infectious bronchitis (IB)
- -> chicken – respiratory signs
- -> hens – egg deformities
- -> nephropathogen strains – nephritis, gout
- Turkey bluecomb disease
- -> enteritis, diarrhea
- -> exsiccosis, cyanosis
- Duck, Goose, Pigeon, Pheasant coronaviruses
Deltacoronavirus genus:
(Nidovirales, Coronaviridae, Orthocoronavirinae)
- Wild bird (bulbul, trush) coronaviruses
Tobaniviridae:
- subfamily, genera
(Nidovirales)
- Torovirinae: Torovirus genus:
- Piscanivirinae alcsalád: Bafinivirus genus:
Torovirus genus:
(Nidovirales, Tobaniviridae, Torovirinae)
- Berne virus
- -> foal gastroenteritis
- Breda virus
- -> calf gastroenteritis
- Chicken, swine, human gastroenteritis
Bafinivirus genus
(Nidovirales, Tobaniviridae, Piscanivirinae alcsalád)
- White bream virus
Arteriviridae:
- subfamily, genus
(Nidovirales)
- Equarterivirinae: Alphaarterivirus genus
- Variarterivirinae: Betaarterivirus genus
( - Simarterivirinae – Deltaarterivirus
?)
Arteriviridae:
- Morphology
- Biological properties
- Antigenic properties
- Diagnosis
Morphology: - +ssRNA genome - ~60nm icosahedral capsid - enveloped – 70nm Resistance: weak (37°C, pH 6-8)
Biological properties:
- Baltimore IV., subgenomic mRNA synthesis
- CPE: not typical or no
- Transmission: sexual route, aerogen, contact
Antigenic properties:
- good antigens, no cross reactions
Diagnosis:
- clinical signs, isolation (difficult) histopathology, IF, RT-PCR
- indirect: VN, ELISA
Alphaarterivirus genus:
(Nidovirales, Arteriviridae, Equarterivirinae)
- Equine arteritis virus (EAV)
–> arteritis – edema, abortion, respiratory signs
–> stallions may become persistently infected
carry the virus in the genital tract
shed the virus by the semen
Betaarterivirus genus
(Nidovirales, Arteriviridae, Variarterivirinae)
- Porcine reproductive and respiratory syndrome (PRRS)
- -> (Swine infertility and respiratory syndrome – SIRS)
- -> early abortion – infertility
- -> respiratory signs – cyanosis (blue ear)
Deltaarterivirus:
(Nidovirales, Arteriviridae, Simarterivirinae)
- Monkey hemorrhagic fever virus
