17a+b: asfar-hepadna, parvo, circo Flashcards
Asfaraviridae
- genus
(African swine fever)
- Asfivirus genus
Asfarviridae
- Morphology
- Biological properties
- Antigenic properties
- diagnosis
Morphology:
- linear dsDNA
- ~250nm icosahedral capsid
- enveloped
Resistance: strong
- In raw pork meat the virus survives for months!
Biological properties:
- Baltimore I. ↔ multiplication in the cytoplasm (viral enzymes)
- multiplication in lymphatic cells – buffy coat culture
- haemadsorption
- stenoxen
- reservoir (Africa): wart-hog
- transmission: contact, arbo (ticks!)
Antigenic properties:
- hidden protective antigen – neutralizing antibodies emerge in late stage of infection!
- no vaccines available
- persisting infections
- immunocomplex deposition – clinical signs
Diagnosis:
- clinical signs = classical swine fever – swine experiment
- isolation – buffy coat
- IF, ELISA
Asfivirus genus:
(Asfarviridae) African swine fever - swine – high morbidity and mortality - acute: fever, haemorrhage (skin, organs) - chronic: paralysis, wasting
Hepadnaviridae:
- genera
- Orthohepadna-, Avihepadnavirus genus
Hepadnaviridae:
- Morphology
- Biological properties
- Antigenic properties
- diagnosis
Morphology: - circular ss/dsDNA - ~ 45 nm icosahedral capsid - enveloped Resistance: weak (low pH, detergents)
Biological properties:
- Baltimore VI! – replication with reverse transcriptase
- stenoxen viruses
- transmission: blood, sexual route, close contact
- chronic, persisting infections
Antigenic properties:
- HBsAg - protective, HBcAg, HBeAg
Diagnosis:
- ELISA direct and indirect
Orthohepadnavirus genus:
(Hepadnaviridae)
- Human hepatitis B
- -> serumhepatitis
- -> hepatocarcinoma
- woodchuck hepatitis
- ground squirrel hepatitis
- bat hepatitis
- monkey hepatitis
Avihepadnavirus genus:
(Hepadnaviridae)
- Duck hepatitis (B)
- -> Far East
- -> hepatitis
- heron, parrot hepatitis B viruses
Parvoviridae:
- Subfamilies, genera:
- Parvovirinae: Proto-, Avi-, Amdo-, Boca-, Copi-, Tetra-, Erythro-, Dependoparvovirus genus
- Densovirinae
Parvoviridae:
- Morphology
- Biological properties
- Antigenic properties
- diagnosis
Morphology:
- linear ssDNA – self-priming, hairpin ends
- ~20nm icosahedral capsid
- non enveloped
Resistance: very strong (60°C, 60 min; pH 3-9)
Biological properties:
- Baltimore II.
- -> cellular polymerase - multiplication in dividing cells
- -> complementation – adeno- or herpesvirus infected cells
- hemagglutination
- stenoxen
Antigenic properties:
- very good antigens – long immunity
- cross reactions – cross immunity
→ related viruses (mutants) – panleukopenia, mink enteritis, canine parvovirus 2
Diagnosis:
- isolation in dividing cells – intranuclear inclusion bodies
- HA, IF, AGID
- Serology: HAI, VN
Protoparvovirus genus:
(Parvoviridae, Parvovirinae)
- Porcine parvovirus – SMEDI
→ Stillbirth, Mummification, Embryonic Death, Infertility
- Canine parvovirus 2 – enteritis, myocarditis, exsiccosis
- Feline panleukopenia virus – panleukopenia
- Mink enteritis virus
Aviparvovirus genus:
(Parvoviridae, Parvovirinae)
- Chicken parvovirus – stunting, diarrhoea, mortality
Amdoparvovirus genus:
(Parvoviridae, Parvovirinae)
- Aleutian mink disease virus
- -> immunocomplex disease – hemorrhage, nephritis
Bocaparvovirus genus:
(Parvoviridae, Parvovirinae)
- Bovine parvovirus
- Canine minute virus (CPV-1)
Erythroparvovirus genus:
(Parvoviridae, Parvovirinae)
- B 19 virus
human transient aplastic crisis, fetal wastage (hydrops)
- Simian erythroviruses
Dependoparvovirus genus:
(Parvoviridae, Parvovirinae) - Adeno associated viruses (human, canine, bovine, ect.) - Goose parvovirus – Derzsy’s disease ascites, respiratory disease, enteritis - Muscovy duck parvovirus - Snake parvovirus
Copi-, Tetraparvovirus genus:
(Parvoviridae, Parvovirinae)
- ungulate parvoviruses
Densovirinae:
(Parvoviridae)
(densonucleosis virus)
- arthropod pathogens
Circoviridae:
- Genera
Circo-, Cyclovirus genus
Circoviridae:
- Morphology
- Biological properties
- Antigenic properties
- diagnosis
Morphology: - circular ssDNA - ~20nm icosahedral capsid - non enveloped Resistance: very strong (60°C, 60 min; pH 3-9)
Biological properties:
- Baltimore II.
–> cellular polymerase - multiplication in dividing cells
→ bone marrow cells, leukocytes - immunosuppressant
- germinative infections
- stenoxen
Antigenic properties: good antigens ↔ immunosuppression
Diagnosis:
- isolation: dividing cells, embryo
- IF (leukocytes)
- Serology: ELISA
Circovirus genus:
(Circoviridae)
- Porcine circovirus – PCV-1; PCV-2: PMWS, PDNS
- -> wasting, hemorrhage, immunosuppression
- Beak and feather disease virus
- -> parrots
- -> beak and feather deformities, immunosuppression
- Goose circovirus
- -> immunosuppression
- Canary, pigeon, mallard duck, Muscovy duck circoviruses
PCV-2:
(Circoviridae, Circovirus genus: Porcine circovirus)
- Post-weaning Multisystemic Wasting Syndrome (PMWS)
- Porcine Dermatitis and Nephropathy Syndrome (PDNS)
- -> wasting, hemorrhage, immunosuppression
Anelloviridae:
- genera
Gyrovirus, Alpha-Zetatorquevirus
Anelloviridae:
- Morphology
- Biological properties
- Antigenic properties
- diagnosis
Morphology: - circular ssDNA - ~20nm icosahedral capsid - non enveloped Resistance: very strong (60°C, 60 min; pH 3-9)
Biological properties:
- Baltimore II.
–> cellular polymerase - multiplication in dividing cells
→ bone marrow cells, leukocytes - immunosuppressant
- germinative infections
- stenoxen
Antigenic properties: good antigens ↔ immunosuppression
Diagnosis:
- isolation: dividing cells, embryo
- IF (leukocytes)
- Serology: ELISA
- PCR
Gyrovirus genus:
(Anelloviridae)
- Chicken infectious anemia
- -> anemia, immunosuppression
Alpha-Zetatorquevirus genus:
(Anelloviridae)
Torque teno virus
- human, swine, horse, dog, cat, seal…
- asymptomatic infection (associated to other diseases)
- 100% seropositivity, life long viraemia
