14: poly, papi, adeno

Question 1

Classes in the Baltimore system:

Answer 1

• Ordo (order): -virales
• Family: -viridae
• Subfamily: -virinae
• Genus: -virus
• Serotype (=Species)
• Subtype, strain

Question 2

Polyomaviridae

• Morphology
• Biological properties
• Diagnosis

Answer 2

Morphology:
- circular dsDNA, superhelical
- ~ 40 nm icosahedral capsid
- no envelope
Resistance: strong (56°C, 30 min; pH 3, lipid solvents)

Biological properties:

• Baltimore I. – cellular transcriptase
• oncogenic – SV40 model virus
• latency: integration into the genome – provirus
• persistent infection in the kidney – viruria
• air borne
• in cell cultures – microtumors
• haemagglutination

Diagnosis:

• IF, EM, HAI
• NA-hybridization, PCR

Question 3

Polyomaviridae:

- Genus(es):

Answer 3

• Polyomavirus (alpha-deltapolyomavirus

Question 4

Polyomavirus genus:

Answer 4

Murine polyomavirus (laboratory mice)
Simian polyomavirus SV40 (monkey ↔ rodents tumors)
Bovine polyomavirus
Goose polyomavirus – nephritis
Budgerigar fledgling disease virus

Question 5

Papillomaviridae

• Morphology
• Biological properties
• Antigenic properties
• Diagnosis

Answer 5

Morphology:
- circular dsDNA, superhelical
- ~ 55 nm icosahedral capsid
- no envelope
Resistance: strong (56°C, 30 min; pH 3, lipid solvents)

Biological properties:

• Baltimore I. – cellular transcriptase
• virus assembly in keratinized cells
• -> no propagation in tissue cultures
• stenoxen (restricted host range, predilection sites)
• oncogenic – benignant tumors
• latency: episomal DNA
• contact, venereal transmission – cell-associated

Antigenic properties:
- good antigens ↔ hidden for the immune system
→ autovaccines
- no cross reactions

Diagnosis:

• Clinical signs, IF, EM
• NA hybridization

Question 6

Papillomaviridae

• subfamily
• genus

Answer 6

Subfamily: First-, Secondpapillomavirinae
Genus: Papillomavirus (38, α-Ω +)

Question 7

Papillomavirus genus:

Answer 7

warts, papillomas: benignant tumors – pre-malignant status
- Equine papillomatosis – head, nose
transmission: bit, twitch
- Bovine papillomatosis 1-13 – neck, back, body
horse sarcoid
- Canine oral papillomatosis – oral mucosa, epulis
- Sheep, roe deer, elephant, elk, possum, rabbit, turtle, parrot…
- Human 1-63
- Skin papillomas – verruca vulgaris
+ autosomal disorders and environmental factors
→ squamosus cell carcinomas
- Genital papillomas – sexually transmitted disease
Juvenile laryngeal carcinomas
Cervical intraepithelial neoplasia
Vulval-, penile carcinomas

Question 8

Adenoviridae

- genus:

Answer 8

Mastadeno-, Aviadeno-, Atadeno-, Siadeno- Ichtadenovirus

Question 9

Adenoviridae

• Morphology
• Biological properties
• Antigenic properties
• Diagnosis

Answer 9

Morphology:
- linear dsDNA
- ~ 80 nm icosahedral capsid, + fibres (HA activity)
- no envelope
Resistance: strong (pH 3, iodophores Ø, Aviadeno: 56°C, 30 min)

Biological properties:

• Baltimore I. – cellular transcriptase
• stenoxen (except atadenoviruses, CAV-1)
• CPE: nuclear inclusion bodies, rounding, lysis
• oncogenity – apoptosis inhibition

Antigenic properties:

• good antigens
• cross reactions – AGID, CF, IF
• serotyping: VN, HAI

Diagnosis:

• isolation
• AGID, VN

Question 10

Mastadenovirus genus:

Answer 10

(Adenoviridae)

• Pneumoenteritis of calves (BAV 1-3, 9)
• Pneumoenteritis of lambs (OAV 1-6), bladder stone
• Infectious canine hepatitis – Fox encephalitis (CAV-1)
• Canine laryngotracheitis (CAV-2) – kennel cough
• Human adenoviruses (HAV 1-41) – military disease

Question 11

Aviadenovirus genus:

Answer 11

(adenoviridae)

• Chicken (12), turkey (2), goose (3), pigeon adenoviruses
• Inclusion body hepatitis, nephritis, gizzard erosion
• CELO, GAL

Question 12

Atadenovirus genus:

Answer 12

(adenoviridae)

• Pneumoenteritis of calves (BAV 4-8, 10) and lambs
• Egg drop syndrome (EDS)
• Snake adenoviruses

Question 13

Siadenovirus genus:

Answer 13

(adenoviridae)

• Hemorrhagic enteritis of turkey / Marble spleen disease of pheasant
• Frog adenoviruses

Question 14

Ichtadenovirus genus:

Answer 14

(adenoviridae)

- Sturgeon adenovirus