17.5.6 - Peptides and Proteins Flashcards
1
Q
What type of reaction joins amino acids together to form a peptide bond?
A
Condensation reaction
2
Q
When hydrolysing a peptide, what conditions are required?
A
Heating with concentrated HCl
and water
3
Q
When you hydrolyse a peptide into amino acids, what happens to the NH2 groups?
A
It is protonated to form NH3+ groups
4
Q
What is the developing agent used to make amino acids glow on chromotography paper?
A
Ninhydrin
5
Q
How can different amino acids be separated and identified?
A
Hydrolysis of a peptide
Use chromotography with ninhydrin
Measure Rf value for each amino acid.
6
Q
Are amino acids separated by an electrical field or by mass?
A
Electric field.
