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MT6320 > 03: Non-Fermenting Gr Neg > Flashcards

03: Non-Fermenting Gr Neg Flashcards

1
Q

Pseudomonas is oxidase ___

A

positive

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2
Q

Acinetobacter and Stenotrophomonas are oxidase ___

A

negative

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3
Q

Non-fermenting Gr (-) bacteria are motile except ___

A

Burkholderia mallei

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4
Q

Oxygen requirement of non-fermenters

A

obligate aerobes

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5
Q

Most important spp. in the fluorescent group

A

P. aeruginosa

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6
Q

Fluorescent group

Leading cause of nosocomial respiratory tract infection

A

P. aeruginosa

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7
Q

Fluorescent group

Causes Otitis externa on swimmer’s or diver’s infection

A

P. aeruginosa

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8
Q

Fluorescent group

Cause of Jacuzzi/ hot tub syndrome

A

P. aeruginosa

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9
Q

Fluorescent group

Cause of bacteremia leading to ecthyma gangrenosum

A

P. aeruginosa

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10
Q

Fluorescent group

Virulence factor of P. aeruginosa that makes it resistant to most antibiotics

A

unusual porin

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11
Q

Virulence factor of P. aeruginosa

inhibits/blocks protein synthesis

A

exotoxin A

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12
Q

Virulence factor of P. aeruginosa

Polysaccharide polymer in mucoid strains

A

alginate

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13
Q

Fluorescent group

Pigments produced by P. aeruginosa

A
  • Pyocyanin - blue, water-soluble
  • Pyoverdin - yellow green, fluorescein
  • Pyorubin - red
  • Pyomelanin - brown
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14
Q

Fluorescent group

Odor produced by P. aeruginosa

A

fruity or “corn tortilla-like” odor

2-aminoacetophenone

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15
Q

Fluorescent group

Optimal temperature for P. aeruginosa growth

A

42C

stenotrophomonas

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16
Q

Fluorescent group

Exclusive growth media for P. aeruginosa

A

Cetrimide agar

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17
Q

Fluorescent group

Colony morphology of P. aeruginosa in BAP

A

ground glass appearance with feathery edge

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18
Q

Fluorescent group

Hemolytic pattern of P. aeruginosa

A

B-hemolytic

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19
Q

Fluorescent group

Appearance of P. aeruginosa colonies on colorless medium

A

bluish-green, diffusable pigment

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20
Q

Fluorescent group

Other members of the fluorescent group that has a very low virulence, rarely causing clinical disease

A

P. fluorescens & P. putida

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21
Q

Other member of fluorescent group aside from P. aeruginosa

Associated with catheter-related sepsis in patients with cancer

A

P. putida

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22
Q

Other member of fluorescent group aside from P. aeruginosa

Infections related to contaminated catheters and catheter-related devices

A

P. fluorescens

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23
Q

Fluorescent group

Nitrate reducton and acetamide utilization reaction of P. fluorescens and P. putida

A

negative, negative

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24
Q

Fluorescent group

Pigment produced by P. fluorescens and P. putida

A

Pyoverdin

they do not produce pyocyanin

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25
Q

Fluorescent group

Method of differentiation of P. fluorescens and P. putida

A

Gelatin hydrolysis

  • P. fluorescens (+)
  • P. putida (-)
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26
Q

Non-fluorescent group

Easily recognizable, causes otitis media, conjunctivitis, septic arthritis in cystic fibrosis patients and meningitis and UTI in HIV patients

A

Pseudomonas/ Burkholderia stutzeri

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27
Q

Non-fluorescent group

Causes endocarditis and is a usual contaminant

A

P. mendocina

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28
Q

Non-fluorescent group

Dry, wrinkled, leathery colonies that produce light yellow or buff/light brown color

A

P. stutzeri

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29
Q

Non-fluorescent group

Arginine dihydrolase (AD) and starch hydrolysis (SH) reaction of P. stutzeri

A

AD (-)
SH (+)

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30
Q

Non-fluorescent group

Often considered contamnant when isolated from clinical specimens

A

P. pseudoalcaligenes & P. alcaligenes

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31
Q

Non-fluorescent group

Oxidase reaction of P. pseudoalcaligenes and P. alcaligenes

A

positive

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32
Q

Non-fluorescent group

Differentiating test between P. pseudoalcaligenes and P. alcaligenes

A

ADH

  • P. pseudoalcaligenes (-)
  • P. alcaligenes (+)

P. pseudoalcaligenes may also weakly ferment fructose

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33
Q

Non-fluorescent group

P. pseudoalcaligenes and P. alcaligenes are motile due to ___

A

polar flagella

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34
Q

Acinetobacter spp.

The two species most commonly seen in clinical specimens

A

A. baumannii & A. lwoffii

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35
Q

Acinetobacter spp.

Glucose-oxidizing, nonhemolytic strain

A

A. baumannii

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36
Q

Acinetobacter spp.

Glucose negative, nonhemolytic strain

A

A. lwoffii

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37
Q

Acinetobacter spp.

Much less virulent, and when isolated, usually indicates contamination or colonization rather than infection

A

A. lwoffii

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38
Q

Acinetobacter spp.

Implicated in meningitis among immunocompromised

A

A. lwoffii

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39
Q

Associated with ventilators, humidifiers, catheters, etc.

A

Acinetobacter spp.

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40
Q

Catalase and oxidase reaction of Acinetobacter spp.

A

catalase (+)
oxidase (-)

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41
Q

Motility of Acinetobacter spp.

A

non-motile

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42
Q

Acinetobacter spp.

B-hemolytic strain

A

A. haemolyticus

43
Q

Acinetobacter spp.

Colonies of A. baumanni in EMB

A

purplish hue with a blue-grey (cornflower blue) center

due to lactose oxidation

44
Q

Acinetobacter spp.

Large, gummy, translucent, gray white colonies in BAP

A

A. baumanni

45
Q

Acinetobacter spp.

Colonies of A. baumanni in MAC

A

Bluish/ peach to pink color

46
Q

3rd most commonly isolated among non-fermenters

A

Stenotrophomonas maltophilia

47
Q

Stenotrophomonas spp.

Most common source of S. maltophilia contamination

A

blood drawing equipment

48
Q

Stenotrophomonas spp.

Oxidase reaction of S. maltophilia

A

negative

49
Q

Stenotrophomonas spp.

Colony appearance of S. maltophilia on MAC

A

bluish

50
Q

Stenotrophomonas spp.

Colony appearance of S. maltophilia on BAP

A

lavender green pigment

51
Q

Stenotrophomonas spp.

CHO oxidation of S. maltophilia

A
  • glucose: w (+)
  • maltose: s (+)
52
Q

Burkholderia spp.

Isolated from irrigation fluids, anesthetics, nebulizers, detergents, and disinfectants

A

B. cepacia

53
Q

Burkholderia spp.

Has an onion bulb rot in plants and food rot in humans odor

A

B. cepacia

54
Q

Burkholderia spp.

Colony appearance and odor of B. cepacia on BAP

A

smooth and slightly raise; dirt-like odor

55
Q

Burkholderia spp.

Appearance of B. cepacia on MAC

A

colorless; becomes pink to red after 4-7 days

56
Q

Burkholderia spp.

Most strains are ONPG ___

A

positive

57
Q

Burkholderia spp.

Specific selective media for B. cepacia

A
  • PC (Pseudomonas cepacia Agar)
  • OFPBL
  • BCSA (Burkholderia cepacia Selective Agar)
58
Q

Burkholderia spp.

Pigment produced by B. cepacia

A

Nonfluorescing yellow/green pigment

59
Q

Burkholderia spp.

Causes melioidosis

A

B. pseudomallei

60
Q

Burkholderia spp.

Found in water and muddy soils in Southeast Asia including Vietnam and Thailand

A

B. pseudomallei

61
Q

Burkholderia spp.

Etiologic agent of Vietnam Time Bomb

A

B. pseudomallei

62
Q

Burkholderia spp.

MOT of B. pseudomallei

A

direct innoculation

63
Q

Burkholderia spp.

Specific selective media for B. pseudomallei

A

Ashdown media

64
Q

Burkholderia spp.

Colony appearance of B. pseudomallei on BAP

A

smooth to wrinkled

65
Q

Burkholderia spp.

Appearance of B. pseudomallei in gram staining

A

has bipolar, safety pin appearance

66
Q

Burkholderia spp.

Colony appearance of B. pseudomallei on Ashdown media

A

dry, wrinkled pink or purple

67
Q

Burkholderia spp.

Distinct odor of B. pseudomallei

A

earthy odor

68
Q

Burkholderia spp.

CHO oxidized by B. pseudomallei

A
  • glucose
  • maltose
  • lactose
  • mannitol

P. stutzeri doesn’t oxidize lactose w/ch is its differentiating factor

69
Q

Burkholderia spp.

Associated in patients with cystic fibrosis and chronic granulomatous disease px.

A

B. glandioli

70
Q

Burkholderia spp.

Causes Glander’s/Farcy disease

A

B. mallei

71
Q

Burkholderia spp.

Disease caused by B. mallei that is a respiratory infection in livestocks and horses

A

Glander’s/ Farcy disease

72
Q

TSI reaction of P. aeruginosa

A

K/K

73
Q

Laboratory dx. (non-fermenting gr neg bacilli)

Determine the ability of an organism to use acetamide as the sole source of carbon

A

acetamide utilization

only P. aeruginosa will be (+)

74
Q

Laboratory dx. (non-fermenting gr neg bacilli)

Indicator of acetamide utilization test

A

bromthymol blue

turns acetamide to ammonia, resulting in blue color

75
Q

Laboratory dx. (non-fermenting gr neg bacilli)

Spp. that will have good growth at 42C and 35C

A
  • P. aeruginosa
  • S. maltophilia
76
Q

Laboratory dx. (non-fermenting gr neg bacilli)

Will not present good growth at 42C and 35C

A
  • P. fluorescens
  • P. putida
77
Q

Less commonly encountered non-fermenting gr neg bacilli

Found in water

A

A. faecalis

78
Q

Less commonly encountered non-fermenting gr neg bacilli

A. faecalis is motile due to ___

A

peritrichous flagella

79
Q

Less commonly encountered non-fermenting gr neg bacilli

Most commonly isolated Achromobacter spp.

A

A. xylosoxidans

80
Q

Less commonly encountered non-fermenting gr neg bacilli

Odor and colony appearance of Achromobacter on SBA

A

fruity odor; green discoloration

81
Q

Less commonly encountered non-fermenting gr neg bacilli

Can be isolated in an immunocompromised px.

A

Brevundimonas spp.

82
Q

Less commonly encountered non-fermenting gr neg bacilli

Motile isolates of Brevundimonas spp.

A

B. diminuta

83
Q

Less commonly encountered non-fermenting gr neg bacilli

In Brevundimonas spp., this virulence factor contributes to the multidrug resistance

A

VIM-2metallo-B-lactamase

84
Q

Less commonly encountered non-fermenting gr neg bacilli

Recently considered to be a pathogen

A

Brevundimonas spp.

85
Q

Less commonly encountered non-fermenting gr neg bacilli

On gram stain, this organism may appear as curved bacilli that resembe vibrios, with slightly curved gram neg rodes with rounded ends

A

C. violaceum

86
Q

Less commonly encountered non-fermenting gr neg bacilli

Solubility of violacein in water and ethanol

A

water-insoluble
ethanol-soluble

87
Q

Less commonly encountered non-fermenting gr neg bacilli

Appearance of C. violaceum in MHA

A

black or very dark purple

88
Q

Less commonly encountered non-fermenting gr neg bacilli

Odor of C. violaceum in SBA

A

ammonium cyanide

89
Q

Less commonly encountered non-fermenting gr neg bacilli

Can be isolated from swimming pools, as well as hospital equipment and laboratory supplies

A

S. paucimobilis

90
Q

Less commonly encountered non-fermenting gr neg bacilli

The yellow pigment of S. paucimobilis only appears on ___ and not on MAC, and requires ___ hours

A

SBA, 48 hours

91
Q

Less commonly encountered non-fermenting gr neg bacilli

S. paucimobilis is motile at ___ to ___, but not at 37C

A

10-22

92
Q

Less commonly encountered non-fermenting gr neg bacilli

Flavobacteriaceae are nonmotile except ___

A

B. alpaca

93
Q

Less commonly encountered non-fermenting gr neg bacilli

All Flavobacteriaceae are indole positive except ___

A

Myroides & Sphingobacterium

94
Q

Less commonly encountered non-fermenting gr neg bacilli

All Flavobacteriaceae are polymyxin B resistant except ___

A

W. virosa

polymyxin B (S)

95
Q

Flavobacteriaceae

Cause meningitis or septicemia in a newborn, especially in conjunction with prematurity

A

Elizabethkingia meningoseptica

96
Q

Flavobacteriaceae

Curve to straight rod, motile by polar flagella

A

B. alpaca

97
Q

Flavobacteriaceae

Appearance of B. alpaca colonies

A

pale yellow, becoming brown with age

98
Q

Flavobacteriaceae

Most frequently isolated species found in human specimens

A

C. indologenes

99
Q

Flavobacteriaceae

This spp. is insignificant when isolated in rare numbers or from only one medium or one specimen

A

C. indologenes

100
Q

Shewanella spp.

From environmental sources such as stagnant water, natural gas, brine, and spoiled dairy products

A

S. putrefaciens

101
Q

Shewanella spp.

More frequently isolated from clinical specimens

A

S. algae

102
Q

Shewanella spp.

Appearance of Shewanella colonies on SBA

A

tan to brown pigment causing greenish coloration

these colonies are often mucoid

103
Q

Shewanella spp.

Test to differentiate Shewanella from Enterbactericeae

A

Oxidase test

  • Shewanella (+)
  • Enterobacteriaceae (-)
104
Q

Shewanella spp.

S. algae requires ___ and is asaccharolytic

A

NaCl

S. putrefaciens is nonhalophilic and saccharolytic

MT6320 (10 decks)

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