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MT6320 > 03: Haemophilus & Other Fastidious Bacteria 1 > Flashcards

03: Haemophilus & Other Fastidious Bacteria 1 Flashcards

1
Q

Most Haemophilus spp. are oxidase positive except ___

A
  • H. ducreyi
  • A. aprophilus
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2
Q

Most Haemophilus spp. are catalase positive except ___

A
  • H. ducreyi
  • A. sequis
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3
Q

happy

A

studying :)

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4
Q

Most Haemophilus spp. require X and V factors except ___

A
  • H. aprophilus/ A. aprophilus
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5
Q

Most Haemophilus spp. inhabit URT and oral cavity except ___

A

H. ducreyi

causes STD (chanchroid)

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6
Q

Most Haemophilus spp. are normal flora and opportunistic pathogens except ___

A
  • H. influenzae
  • H. ducreyi

they are encapsulated

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7
Q

Haemophilus spp.

Common name of H. influenzae

A

Pfeiffer’s Bacillus

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8
Q

Haemophilus spp.

T/F: H. influenzae causes influenza

A

F

name is a misnomer, but it is associated with flu during pandemic

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9
Q

Haemophilus spp.

H. influenzae may be recovered in ___

A

upper RT (oropharynx)

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10
Q

Virulence factor of H. influenzae

Basis of serotyping

A

Capsule

made up of polyribosyl phosphate (PRP)

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11
Q

Virulence factor of H. influenzae

Cleave IgA

A

IgA protease

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12
Q

Most virulent H. influenzae serotype and is commonly associated with patient with meningitis

A

b

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13
Q

H. influenzae biotype is based on ___

A
  • indole
  • urease
  • ODC
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14
Q

H. influenzae biotype

Meningitis and septicemia

A

biotype I

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15
Q

H. influenzae biotype

  • Indole (+)
  • Urease (+)
  • ODC (+)
A

biotype I

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16
Q

H. influenzae biotype

Eye and RT infections

A

biotype II, III

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17
Q

H. influenzae biotype

  • Indole (-)
  • Urease (+)
  • ODC (-)
A

biotype III

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18
Q

Rx. for H. influenzae

A

ceftriaxone or cefotaxime

rifampin for chemoprophylaxis

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19
Q

H. influenzae strain

Causes meningitis, epiglottitis, bacterial tracheitis

A

capsulated strains

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20
Q

H. influenzae strain

Causes otitis media, conjunctivitis, sinusitis, bacteremia, pneumonia

A

non-capsulated strains

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21
Q

Haemophilus spp.

A strict human pathogen that is present in female sexual workers

A

H. ducreyi

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22
Q

Haemophilus spp.

Common name for H. ducreyi

A

Ducrey’s Bacillus

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23
Q

Rx. for H. ducreyi

A
  • azithromycin
  • ceftriaxone
  • ciprofloxacin
  • erythromycin
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24
Q

Haemophilus spp.

Causes genital ulcer disease in the penis, labia, and vagina

A

H. ducreyi

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25
Q

Haemophilus spp.

Causes suppurative buboes

A

H. ducreyi

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26
Q

Haemophilus spp.

Causes pediatric conjunctivities and Brazilian purpuric fever

A

H. influenzae biogroup aegyptius

or H. influenzae biogroup III; resembles H. aegyptius

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27
Q

Haemophilus spp.

Common name for H. aegyptius

A

Koch-Weeks Bacillus

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28
Q

Haemophilus spp.

Causes pink-eye, a highly contageous conjunctivitis

A

H. aegyptius

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29
Q

Haemophilus spp.; opportunistic pathogens

Causes endocarditis

A

A. aprophilus

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30
Q

Haemophilus spp.; opportunistic pathogens

Causes mitral valve endocarditis

A

H. parainfluenzae

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31
Q

Haemophilus spp.; opportunistic pathogens

Causes pharyngitis

A

H. parahaemolyticus

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32
Q

Haemophilus spp.

Transport media

A
  • Amie’s with charcoal
  • Modified Stuart’s
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33
Q

Haemophilus spp.; appearance in gram staining

Gr (-) small coccobacilli or small rods

A

H. influenzae

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34
Q

Haemophilus spp.; appearance in gram staining

Long slender rods

A

H. influenzae biotype aegyptius

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35
Q

Haemophilus spp.; appearance in gram staining

School of fish

A

H. ducreyi

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36
Q

Haemophilus spp.; appearance in gram staining

Very short coccobacilli

A

H. aprophilus

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37
Q

Haemophilus spp.

Preferred isolation medium

A

CAP

35-37C; 24 hrs; increased CO2; + bacitracin enriched w/ 1% IsoVitalex

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38
Q

Haemophilus spp.

Used for primary isolation

A

Staph Streak

allows satellite growth; S. aureus produces NAD

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39
Q

Haemophilus spp.

A medium used for H. ducreyi isolation

A

Nairobi Biplate

Mueller-Hinton based CAP

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40
Q

Haemophilus spp.

A clear medium with X and V factors

A

Levinthal Agar

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41
Q

Haemophilus spp.

Appearance of colonies of encapsulated H. influenzae in Levinthal Agar

A

iridescent colonies

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42
Q

Haemophilus spp.

Appearance of non-encapsulated H. influenzae in Levinthal Agar

A

transparent, bluish, non-iridescent

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43
Q

Haemophilus spp.

Distinct odor of H. influenzae

A

mousy/ bleach-like odor

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44
Q

Haemophilus spp.

Test for X factor dependence

A

Porphyrin test

(+): red fluorescence

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45
Q

Haemophilus spp.

Serologic test that uses Staphylococcal coagglutination technique

A

Phadebact

46
Q

Haemophilus spp.

Positive result of Neufeld Quellung Reaction

A

Absence of turbidity

47
Q

Haemophilus spp.

Does not ferment CHO

A

H. ducreyi

48
Q

Haemophilus spp.

Ferments glucose, sucrose, lactose, fructose, and ONPG (+)

A

H. aprophilus

49
Q

Haemophilus spp.

Ferments glucose, sucrose, frucose, but not lactose

A
  • H. parainfluenzae
  • H. parahaemolyticus
50
Q

Haemophilus spp.

Does not require X & V factor; grows on SBA, CAP

A

H. aprophilus

51
Q

Haemophilus spp.

Require X factor only; grows on SBA and CAP

A

H. ducreyi

52
Q

Haemophilus spp.

Requires V factor only; grows on CAP only; hemolyses horse/rabbit blood

A

H. parahaemolyticus

53
Q

Haemophilus spp.

Requires V factor only; grows on CAP only; does not hemolyse horse/rabbit blood

A

H. parainfluenzae

54
Q

Catalase and oxidase reaction of P. multocida

A

positive, positive

55
Q

Distinct odor of P. multocida

A

mushroom smell/ musty odor

56
Q

P. multocida appearance in bipolar staining

A

safety pin appearance

57
Q

Hemolytic pattern of P. multocida in SBA

A

non-hemolytic

58
Q

Reservoir of P. multocida

A

oral cavity of domestic dogs, cats, pigs, horses, cattle

59
Q

Causes shipping fever among cattle and RT and wound infections in humans

A

P. multocida

60
Q

Hemorrhagic septicemic fever in animals caused by P. multocida

A

Shipping fever

61
Q

Virulence factor of P. multocida

A
  • endoxotin
  • capsule
62
Q

Brucellaceae

Common name for Brucella

A

Bang’s Bacillus

63
Q

Brucellaceae

Gram staining appearance of Brucella

A

Gram (-) bacilli/ coccobacilli “sand appearance”

64
Q

Catalase and oxidase reaction of Brucella

A

positive, positive

65
Q

Induces spontaneous abortions among animals

A

Brucella

66
Q

Systemic disease caused by Brucella in humans

A

Brucellosis, otherwise known as:

  • Undulant fever
  • Malta fever
  • Bang’s disease
  • Gibraltar fever
  • Mideterranean fever
67
Q

Brucellaceae

B. abortus requires ___ for growth

A

niacin

68
Q

Brucellaceae; reservoir

Cow; camels, yak, buffalo

A

B. abortus

69
Q

Brucellaceae; reservoir

Goat, sheep, camel

A

B. melitensis

70
Q

Brucellaceae; reservoir

Pigs (biotypes 1-3)

A

B. suis

71
Q

Brucellaceae; reservoir

Dogs

A

B. canis

72
Q

Brucellaceae; reservoir

Requires CO2; H2S production (+)

A

B. abortus

73
Q

Brucellaceae

Gold standard for diagnosis

A

detection of febrile agglutinins

paired sera; collected during acute & convalescent; (+) = 4x increase

74
Q

Franciscellaceae

Causes the most severe disease

A

F. tularensis subsp. tularensis

Jellison Type A

75
Q

Franciscellaceae

Less severe; prevalent in Eurasia and North America

A

F. tuleransis subsp. holartica

Jellison Type B

76
Q
A
77
Q

Oxidase and urease reaction of Francisella

A

negative, negative

78
Q

Most Francisella requires cysteine and thiosulfate except ___

A

F. phylomiragia

79
Q

Brucella is classified as a ___ select biological agents by CDC

A

category B

80
Q

Oxidase (+) Francisella spp.

A
  • F. philomiragia
  • F. hispaniensis
81
Q

Francisella is classified as a CDC ___ select biological agent

A

category A

82
Q

Francisella spp.

Minimum infective dose if injected

A

10 organisms

83
Q

Francisella spp.

Minimum infective dose if ingested

A

25 organisms

84
Q

Francisella spp.

Most important reservoir

A

rabbit

85
Q

Francisella spp.; clinical manifestation

Most common

A

ulceroglandular

86
Q

Francisella spp.; clinical manifestation

Most serious form

A

Pneumonic

87
Q

Francisella spp.; clinical manifestation

Obtained after touching eyes or through infective dust

A

oculoglandular

conjunctivitis & lymphadenopathy present

88
Q

DOC for Francisella

A

streptomycin

89
Q

Francisella spp.

Less severe, non-fastidious; causes waterborne tularemia

A

F. philamiragia

90
Q

Francisella spp.

Susceptibility test for tularemia; skin test detecting exposure from the past

A

Foshay test

91
Q

Fastidious spp. from Bordetella genus

A

B. pertussis

92
Q

Bordetella spp.

Catalse and oxidase (+) except ___

A

B. parapertussis

93
Q

Bordetella spp.

Most are non-motile except ___

A
  • B. bronchiseptica
  • B. avium
  • B. hinzii
  • B. ansorpii
94
Q

Bordetella spp.

Common name for B. pertussis

A

Bordet Gengou Bacillus

95
Q

Bordetella spp.

Distinct clinical sign of B. pertussis infection

A

marked lymphocyte increase

96
Q

B. pertussis; virulence factor

Important for adhesion

A

filamentous hemagglutinin and pertactin

97
Q

B. pertussis; virulence factor

Modifies host proteins by ADP ribosyl transferase; stimulate adenylate cyclase

A

Pertussi toxin (exotoxin)

98
Q

B. pertussis; virulence factor

Inhibit epithelial cells and immun effector cells

A

Adenylate cyclase toxin

99
Q

B. pertussis; virulence factor

Desctrution of cilia & promote ciliostasis

A

tracheal cytotoxin

100
Q

DOC for B. pertussis

A

Erythromycin

101
Q

Bordetella spp.

Similar to B. pertussis; causes a less severe respiratory tract infection

A

B. parapertussis

102
Q

Virulence factor of B. parapertussis

A

endotoxin

103
Q

Bordetella spp.

Causes kennel cough among animals

A

B. bronchiseptica

104
Q

Bordetella spp.

Reservoir of B. bronchiseptica

A

dogs & rabbits

105
Q

Appearance of Bordetella spp. in Regan Lowe

A

Mercury drop colonies

106
Q

Incubation time and temperature of Bordetella spp.

A

35C for 7 days

107
Q

Bordetella spp.

  • Catalase (+)
  • Oxidase(+)
  • Nitrate red’n (-)
  • Motility (-)
  • Urease (-)
A

B. pertussis

urease (-) !!

108
Q

Bordetella spp.

  • Catalase (-)
  • Oxidase (-)
  • Nitrate red’n (-)
  • Motility (-)
  • Urease (+); w/in 24 hrs
A

B. parapertussis

catalase & oxidase (-) !!

109
Q

Bordetella spp.

  • Catalase (+)
  • Oxidase (+)
  • Nitrate red’n (+)
  • Motility (+)
  • Urease (+); w/in 4 hrs
A

B. bronchiseptica

motiliy (+) !!

110
Q

Bordetella spp.

  • Brown pigment; beta-hemolytic on Bordet Gengou
  • No growth on Regan Lowe
  • Growth on BAP
  • No growth on MAC
A

B. parapertussis

111
Q

Bordetella spp.

  • Beta-hemolytic on Bordet Gengou
  • No growth on Regan Lowe
  • Growth on BAP
  • Growth on MAC
A

B. bronchiseptica

MT6320 (10 decks)

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